Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional
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2018-06-29
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Universidade Federal de Goiás
Resumo
β-lactamases are enzymes that hydrolyze the β-lactam ring, inactivating the action of β-lactam
antibiotics. The objective of this work was to diagnose phenotypically and molecularly 14 β-
lactamase resistance genes expressed in Pseudomonas aeruginosa and to correlate the results
found. A total of 99 samples of Pseudomonas aeruginosa were selected and the antibiogram
was performed. Real-time PCR is being performed using the Sybr Green system to amplify the
genes corresponding to the resistances found in phenotyping. Three/14 (21.4%) genes
blaSME, blaOXA, blaGIM were found simultaneously in three samples. According to the
statistical test, when evaluating the amplification results obtained for PPT, the molecular
method was more sensitive for the detection of the gene coding for multidrug resistance,
presenting values of (p <0.05). This information suggests that gene research is more sensitive
and specific compared to the antibiogram.
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SANTOS, A. P. Perfil de rastreamento de resistência das Pseudomonas aeruginosa e acompanhamento da rotina educacional. 2018. 114 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2018.