Investigação soroepidemiológica e molecular de brucelose e leptospirose em núcleos de conservação de gado curraleiro pé duro e pantaneiro
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Data
2012-09-21
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Universidade Federal de Goiás
Resumo
The locally adapted breeds to different Brazilian ecosystems characterized as an
alternative to food production, for presenting, among other characteristics, greater
resistance to diseases.The main objective of this study was to evaluate the
epidemiological situation of brucellosis and leptospirosis in nucleus of in situ
conservation of Curraleiro and Pantaneiro bovine breeds in Cerrado and Pantanal
biomes by serological and molecular diagnosis of these diseases in the livestock.
We analyzed serum of 1,280 Curraleiro and 248 Pantaneiro animals on
convenience sampling. We employed microscopic agglutination test, for the
diagnosis of leptospirosis and the buffered acidified antigen test (Rose Bengal plate
test) for brucellosis and 2-mercaptoethanol test for confirmation. The frequency of
brucellosis in Curraleiro was 0.7% for animals and 25% for herds. In Pantaneiros,
all animals tested were negative for Brucella abortus. The frequency of anti-
Leptospira antibodies was 44.8% in Curraleiro and 51.2% in Pantaneiros, and 100%
of the herds of both breeds showed positive animals. The factors associated with
leptospirosis were access to wetland, abortions, vaccination against leptospirosis
and practice of slaughtering in the propertyfor Curraleiro breed; and sex and age,
with the highest risk for females aged between 12 and 24 months for Pantaneiro
breed. Serological methods were employed above and polymerase chain reaction
(PCR) for detecting infection by Brucella spp. and Leptospira spp. in cattle, to
evaluate the concordance of the results of such tests. Brucellosis shows low
frequency in Curraleiro cattle and is controlled in Pantaneiro. Leptospirosis was
widely disseminated in nucleus of in situ conservation. In both breeds the serovars
Hardjo and Wolffi were the most prevalent, which reinforce the function of bovine as
a source of infection in the herd. However, the presence of reactions to serovars
characteristic of wild animals suggests the involvement of local wildlife in the
epidemiology of bovine leptospirosis in these nuclei of in situ conservation. The
correlation of results obtained by serology and PCR to identify infection by Brucella spp. and Leptospira spp. was weak, which reinforces the idea that the diagnosis is
more reliable when more than one method is employed.
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Palavras-chave
Cerrado , diagnóstico , Pantanal , raças bovinas locais , zoonoses , Cerrado , diagnosis , local bovine breeds , Pantanal , zoonosis
Citação
ROMANI, Alana Flávia. Seroepidemiological and molecular monitoring of brucellosis and leptospirosis in nucleus of in situ conservation of curraleiro pé-duro and pantaneiro breeds. 2012. 107 f. Tese (Doutorado em Ciências Agrárias) - Universidade Federal de Goiás, Goiânia, 2012.