Diversidade genética de BGMV em linhagens elite de feijoeiro-comum
Nenhuma Miniatura disponível
Data
2021-06-30
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Brazil is the main common bean consumer in the world, also being the main
producer, whose production supply the intern market. Among the factors that
influence the productivity, the wide distribution of Bean golden mosaic virus in
common bean fields is very important because it can cause chlorosis, leaf
crumpling and stunting, and lead up to 100% yield losses. As a response to
that, Embrapa developed, through the RNAi approach, a transgenic common
bean line with resistance to the Bean golden mosaic virus. Nonetheless, the
presence of a resistance gene can increase the selection pressure over the
pathogen, that can mutate and overcome the resistance. Despite being DNA
viruses, it is described that in species of Begomovirus, the genus of the BGMV,
mutation rates are similar to those of RNA viruses. Nevertheless, until now,
BGMV has shown smaller genetic variability compared to other species of the
genus. The objective of this work was to characterize BGMV populations that
infect different common bean elite lines, transgenic and conventional, in two
locations: Brasília and Santo Antônio de Goiás. To that, leaf samples from
different elite lines were collected in Value for Cultivation and Use (VCU) trials,
searching for differences in viral populations from different sources. We used a
strategy widely used on studies related to other living beings: next generation
DNA sequencing followed by single nucleotide polymorphisms (SNPs)
genotyping. The SNPs obtained were used to carry out genetic diversity
analysis. The estimates of the proportion of polymorphic sites and of Nei’s
gene diversity revealed a greater genetic diversity in viral populations sampled
in transgenic plants. The results suggest that, despite the restriction to the
viral replication and, therefore, of smaller viral populations, the necessary
conditions to the occurrence of selection pressure are given. In the genetic
structure analysis, a significant effect was attributed to localities, as has been
widely described in literature.
Descrição
Palavras-chave
Citação
BERTHOLDO, N. M. Diversidade genética de BGMV em linhagens elite de feijoeiro-comum. 2021. 60 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2021.