Cultivo in vitro e citogenética de Cyrtopodium saintlegerianum Rchb. f.(Orchidaceae: Cyrtopodiinae)
Nenhuma Miniatura disponível
Data
2012-10-30
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
CyrtopodiumsaintlegerianumRchb. f is an epiphytic species typical of the Midwest, especially in
distributed Brazilian Central Plateau, and has a wide geographical distribution in Brazil. It is usually found in the
trunks of palm trees, forming large clumps. It has good features for ornamentation, for the beauty and size of its
inflorescence, however, are not found in the literature about its conservation, methods for its spread or that could be
used in floriculture and landscaping. Thus, this study aimed to establishing protocols for germination asymbiotic
effects of phytohormones and acclimatization and characterization of chromosomal species. In 2010, the establishment
of micropropagation protocols, capsules were collected in a pasture area in the municipality of Mossâmedes, GO, then
the part previously sterilized seeds were separated and tested in a 1% tetrazolium dye.. For cultivation was tested
asymbiotic different culture media and then tested after different concentrations and combinations in treatments BAP
16 / ANA, to finally evaluate the acclimatization substrates combined and fertilized with chemical fertilizers and
organic. For the karyotype of the species, the plant material is derived from plants grown in vitro in culture medium.
We tested four protocols, with differences in enzyme solution for softening the roots, dyes, anti-mitotic concentration
of the solution and hydrolysis, and the use of growth regulators for root induction in vitro. All protocols were in
common roots pretreated with anti-mitotic 8-hydroxyquinoline (0.002 M) in refrigerator for 24 hours. Then protocols
roots were fixed in Carnoy 3:1 for 18 hours the first and second and third and fourth protocol for 24 hours at room
temperature. After stored at -20 º C in the same fixative for further analysis (only the fourth protocol). The roots of the
protocols were stained with different dyes: hematoxylin, Schiff, acetic orcein and Giemsarespectively.The results of
germination was satisfactory in all culture media. For medium supplemented with auxin / cytokinin combined the best
concentrations for variable height were 0.2 mg L-1 NAA and BAP without adding control without addition of
regulators. The best means to induce large numbers of shoots were 4 mg L-1 BAP and 4 mg L-1 BAP / 0.2 mg L-1 NAA.
The number of leaves was rated best in the concentrations of BAP without NAA at concentrations of 1.0, 2.0 and 4.0
mg L-1. The treatments with the highest number of roots were control without added growth regulators at doses of 0.2,
0.5, 1.0 mg L-1 NAA without addition of BAP, as well as the length of roots was favored by the same treatments. The
largest number occurred in callus treatment with concentrations of 1.0 mg L-1 BAP without adding ANA. ForcytogeneticsC. saintlegerianum the best protocol was evaluated with the regulator which was obtained metaphases,
however chromosomes were condensed, and the number of chromosomes was found to be 2n = 48.
cides.
Descrição
Palavras-chave
Citação
SILVA, Daniella Mota. Cultivo in vitro e citogenética de Cyrtopodium saintlegerianum Rchb. f.(Orchidaceae: Cyrtopodiinae). 2012. 84 f. Dissertação (Mestrado em Genética e Melhoramento de Plantas) - Universidade Federal de Goiás, Goiânia, 2012.