Análise da resposta imune celular de pacientes com tuberculose pulmonar ativa contra os antígenos recombinantes MPT-51, GLcB, ESAT-6, Ag 85A e a proteína do filtrado de cultura (CFP) de mycobacterium tuberculosis

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dc.contributor.advisor1KIPNIS, Ana Paula Junqueira
dc.contributor.advisor1Latteshttp://lattes.cnpq.br/1252262903952987por
dc.creatorVASCONCELOS JUNIOR, Arioldo Carvalho
dc.creator.Latteshttp://lattes.cnpq.br/5257261062942900por
dc.date.accessioned2014-07-29T15:30:39Z
dc.date.available2009-06-09
dc.date.issued2008-02-21
dc.description.abstractThis work characterized the specific cellular immune response of TCD4 and TCD8 lymphocytes against recombinant Mycobacterium tuberculosis at the Hospital Anuar Auad, Goiania Brazil, and constituted of two experimental groups: 1) 22 active TB patients with positive acid fast aputum, X-ray indicative of tuberculosis, smear culture positive for M. tuberculosis and HIV negative. 2) 15 sex and age matched healthy controls, tuberculin skin test and HIV negative. Venous blood was drawn and processed to obtain PBMC that were cultivated for 96 hours with the specific antigens (1mg/106 cells). TCD8 and TCD4 cells were analyzed by flow citometry for IL-10 and IFN-g production. In general, the percentage of positive TCD4 and TCD8 cells for IFN-g and IL-10 were superior among the TB patients. Additionally, TCD4+IFNg+ (5,63±2,43) and IL-10+ (5,83± 2,19) cells were significantly higher in TB patients than in healthy controls (TCD4+IFNg+ =1,75±0,71 and IL-10+ =1,47±0,90), (p<0,01). Regarding the percentage of TCD8 cells, a higher percentage of IFNg+ (4,33±1,45) and IL-10+ (4,01±1,14) among TB patients than controls (TCD8+IFNg+ = 1,49±0,42 and IL- 10+ 1,62±0,59) was observed (p<0,01). TB treatment did not alter the response to the tested antigens immediately after the treatment initiation. In conclusion, the recombinant antigens MPT-51, ESAT-6, GLcB, Ag85A were recognized by the specific immune response of active TB patients. Key words: Tuberculosis, Mycobacterium tuberculosis, Recombinant antigens, Cellular immune response. to the tested antigens immediately after the treatment initiation. In conclusion, the recombinant antigens MPT-51, ESAT-6, GLcB, Ag85A were recognized by the specific immune response of active TB patients.eng
dc.description.provenanceMade available in DSpace on 2014-07-29T15:30:39Z (GMT). No. of bitstreams: 1 Dissetacao Arioldo Vasconcelos Junior.pdf: 3999475 bytes, checksum: fcfb183b016528e836128fc94bae53f7 (MD5) Previous issue date: 2008-02-21eng
dc.description.resumoEste trabalho avaliou a resposta imune celular dos linfócitos TCD4 e TCD8 de pacientes com tuberculose pulmonar ativa antes e após o tratamento, contra os antígenos recombinantes MPT-51, ESAT-6, GLcB, Ag85A e o Filtrado Protéico de Cultura (CFP) de Mycobacterium tuberculosis atendidos no Hospital de Doenças Tropicais Anuar Auad. A população de estudo, composta de 37 indivíduos, foi dividida em dois grupos experimentais. 1) 22 pacientes com tuberculose pulmonar, selecionados de acordo com idade, diagnóstico confirmado por baciloscopia (BAAR), radiografia evidente de tuberculose, HIV1/2 negativos. 2) 15 controles saudáveis negativos para prova tuberculinica (PT) e HIV1/2, pareados por faixa etária e sexo aos pacientes selecionados. Coletou-se aproximadamente 20 ml de sangue com heparina. O sangue total foi processado e cultivado por 96 horas na presença dos antígenos recombinantes e os linfócitos TCD4 e TCD8 foram analisados quanto à positividade para as citocinas IL-10 e IFN-g por citometria de fluxo. As porcentagens de linfócitos responsivos aos diversos antígenos de M. tuberculosis apresentaram-se em geral superiores aos controles. A porcentagem dos linfócitos TCD4+IFNg+ (5,63±2,43) e IL-10+ (5,83±2,19) foram maiores em pacientes com tuberculose pulmonar ativa em comparação com os linfócitos TCD4+IFNg+ (1,75±0,71) e IL-10+ (1,47±0,90) dos controles (p<0,01). Também, as porcentagens dos linfócitos TCD8+IFNg+ (4,33±1,45) e IL-10+ (4,01 ±1,14) dos pacientes foram maiores que os linfócitos TCD8+IFNg+ (1,49±0,42) e IL-10+ (1,62±0,59) dos controles (p<0,01). Não houve diferença significativa antes (5,63±2,43) e após (5,0±2,0) o tratamento nas populações celulares TCD4+ e TCD8+ positivas para IFN-g e IL-10 quando estimuladas com o painel de antígenos (p>0,05). Com estes resultados podemos considerar que todos os antígenos testados (MPT-51, ESAT-6, GLcB, Ag85A) são reconhecidos pelos linfócitos TCD4+ e TCD8+ de pacientes com tuberculose pulmonar ativa.por
dc.formatapplication/pdfpor
dc.identifier.citationVASCONCELOS JUNIOR, Arioldo Carvalho. Analysis of Cellular Immune Response Against MPT-51, GLcB, ESAT-6, Ag 85A Recombinant Antigens and the Culture Filtered Protein (CFP) from Mycobacterium tuberculosis from Patients with Active Pulmonary Tuberculosis. 2008. 137 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.por
dc.identifier.urihttp://repositorio.bc.ufg.br/tede/handle/tde/1818
dc.languageporpor
dc.publisherUniversidade Federal de Goiáspor
dc.publisher.countryBRpor
dc.publisher.departmentMedicinapor
dc.publisher.initialsUFGpor
dc.publisher.programMestrado em Medicina Tropicalpor
dc.rightsAcesso Abertopor
dc.subjectTuberculosepor
dc.subjectmycobacterium tuberculosispor
dc.subjectantígenos recombinantespor
dc.subjectresposta imune celularpor
dc.subjectTuberculosiseng
dc.subjectmycobacterium tuberculosiseng
dc.subjectrecombinant antigenseng
dc.subjectcellular immune responseeng
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDE::MEDICINApor
dc.thumbnail.urlhttp://repositorio.bc.ufg.br/TEDE/retrieve/4682/Dissetacao%20Arioldo%20Vasconcelos%20Junior.pdf.jpg*
dc.titleAnálise da resposta imune celular de pacientes com tuberculose pulmonar ativa contra os antígenos recombinantes MPT-51, GLcB, ESAT-6, Ag 85A e a proteína do filtrado de cultura (CFP) de mycobacterium tuberculosispor
dc.titleAnálise da resposta imune celular de pacientes com tuberculose pulmonar ativa contra os antígenos recombinantes MPT-51, GLcB, ESAT-6, Ag 85A e a proteína do filtrado de cultura (CFP) de mycobacterium tuberculosispor
dc.title.alternativeAnalysis of Cellular Immune Response Against MPT-51, GLcB, ESAT-6, Ag 85A Recombinant Antigens and the Culture Filtered Protein (CFP) from Mycobacterium tuberculosis from Patients with Active Pulmonary Tuberculosiseng
dc.title.alternativeAnalysis of Cellular Immune Response Against MPT-51, GLcB, ESAT-6, Ag 85A Recombinant Antigens and the Culture Filtered Protein (CFP) from Mycobacterium tuberculosis from Patients with Active Pulmonary Tuberculosiseng
dc.typeDissertaçãopor

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