Efeitos do laser de baixa potência na leishmaniose experimental: avaliação sobre a lesão cutânea (in vivo) e efeitos sobre leishmânia (in vitro)
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2014-09-01
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Universidade Federal de Goiás
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Leishmaniasis is neglected infectious and parasitic diseases caused by protozoa of the genus Leishmania, having different clinical forms according to the type of host-parasite relationship established, the main ones being: Leishmaniasis Visceral and Cutaneous Leishmaniasis, object of this study. The fact that conventional treatments present limitations and difficulties for patients coupled with increasing use of low level laser therapy in the treatment of skin lesions of various etiologies, specifically with Low power laser, led us to evaluate the possible consequences of such treatment in American cutaneous leishmaniasis in both murine experimental models (in vivo), with the aim of evaluating the dynamics of healing of ulcerated lesions, as in cultured parasites (in vitro), with the use of techniques that allowed us to evaluate the interference of therapy in cultures of the parasites isolated from the MAB-6 Leishmania (Leishmania) amazonensis Leishmanias belonging to the Bank of the Midwest, with regard to the ability to multiply, viability, integrity and infective capacity. The tests "in vivo" concluded that the use of low-power laser is an interesting tool in the treatment of ulcerative lesions caused by cutaneous leishmaniasis since it leads to decreased edema, stimulates microbicidal action of macrophages and promotes the maturation of collagen. Already in essays "in vitro", it was found that the action of the treatment leads to a static effect on the growth curve of the parasites. The viability tests showed a small increase in the number of parasites killed after treatment and reduction in mitochondrial activity. The low level laser therapy proved incapable of promoting the apoptotic process and modify the composition of the plasma membrane of the parasite, which suggests that the laser treatment did not affect the ability of infection. Instead, there was a rise in titers of viable parasites present in the paws of mice infected with Leishmania treated.
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ROCHA, J. G. Efeitos do laser de baixa potência na leishmaniose experimental: avaliação sobre a lesão cutânea (in vivo) e efeitos sobre leishmânia (in vitro). 2014. 99 f. Dissertação (Mestrado em Biologia das Relações Parasito Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2014.