Análise da herança do DNA do Trypanosoma cruzi em parentais e progênies de chagásicos de famílias de São Felipe – Bahia
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2019-02-26
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Universidade Federal de Goiás
Resumo
The Trypanosoma cruzi, etiologic agent of Chagas disease is a flagellate protozoan
transmitted by insect bugs (Reduvidae:Triatomine) to humans and wild mammals. The T.
cruzi contains DNA in the nucleus (nDNA) and in the mitochondrion (kDNA). The kinetoplastid
protozoan comprises maxicircles and and minicircles kDNA network sequences that integrate
in the host’s cell genome. Insertional integrations of kDNA sequences bear high affinity to
retrotransposable LINE-1 randomly distributed in the genome of somatic and germ line cells,
and, thus generate an increasing diversity due to recombination and reshuffling. However, the
profiles of kDNA integrations into the human genome in different Brazilian Ecosystems are
unknown. In this regard, the ratio of transfer of T. cruzi minicircles sequences into the human
genome was south. The study consisted of analyses of 36 members of two families from São
Felipe-Bahia where Chagas disease is endemic. The study showed that 22% (8/36) families’
members had the diagnoses of the disease by immunofluorescence (IF) and enzyme-linked
immunosorbant assay (ELISA). Yet, the PCR assays showed 61% (22/36) of families’
members had kDNA and nDNA assays positive for the T. cruzi infection. In order to detect the
kDNA integration sites in the human genome, a tpTAIL-PCR (Targeted Primer Thermal
Assymetric Interlaced-PCR), which consisted in combination of primers derived from T. cruzi
minicircle conserved sequence with human LINE-1 sequence derived primers, was employed.
The amplicon sequences obtained were subjected to homology analyses in Blast software. The
hybrid kDNA-human DNA sequences revealed lateral and vertical transfer of T. cruzi
minicircles into the genome of Chagas parents and progeny. Unravel of dynamics of mutations
contributes to define variable profiles of kDNA minicircle sequence insertions into the human
genome at different Ecosystems.
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SANTOS, E. J. Análise da herança do DNA do Trypanosoma cruzi em parentais e progênies de chagásicos de famílias de São Felipe – Bahia. 2019. 106 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2019.