CARACTERIZAÇÃO DA ISOCITRATO LIASE E METILISOCITRATO LIASE DO FUNGO PATOGÊNICO HUMANO Paracoccidioides brasiliensis
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Data
2009-02-26
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Universidade Federal de Goiás
Resumo
Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of
paracoccidioidomycosis (PCM), a prevalent systemic mycosis in Latin America.
Pathogenicity appears to be intimately related to the dimorphic transition from the
hyphal to the yeast form, which is induced by a shift from environmental temperature to
the temperature of the mammalian host. To cope with nutrient deprivation during the
infection process, a number of pathogens employ the glyoxylate cycle (GC) to utilize
fatty acids as carbon sources. The genes which constitute this pathway have been
implicated in pathogenesis. An important aspect in the interaction between
P.brasiliensis and your host is the ability to adhere to matrix extracelular components.
In this work has shown that the isocitrate lyase of P. brasiliensis (PbICL) is located in
the cell wall and also in the cytoplasm. PbICL recombinant and polyclonal antibody
were able to inhibit the interaction of P. brasiliensis to epithelial cell cultures in vitro.
Was also evaluated the ability of PbICL recombinant to connect the components of the
extracellular matrix such as laminin, fibronectin, collagen type I and IV. These results
suggest that PbICL is necessary to interaction between molecules of the extracellular
matrix and P.brasiliensis, and that this interaction is crucial in adhesion and invasion of
the fungus to the host cells. The kinetic parameters of PbICLr were determined. The
sequence coding for methylisocitrate lyase of P. brasiliensis (PbmeICL) and the
recombinant protein PbmeICLr were obtained.