Avaliação de diferentes métodos para a classificação de pacientes e de carreadores de antígenos empregados na sorologia de hanseníase
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2014-03-31
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Universidade Federal de Goiás
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Serology to detect IgM antibodies against the PGL-I, a species-specific antigen from Mycobacterium leprae, can be used to aid in the identification of individuals with high bacillary load. Samples and the database from the U-MDT clinical trial were used to compare and report the perfomance of diverse classification methods. This study also evaluated the possibility of enhancement of serological tests by substituting the antigen carrier protein, the bovine serum albumin (BSA) by a human protein (HSA), aiming to eliminate unespecific bindings. The tests specificity was evaluated using sampes from household contacts, healthy population, TB patients and Visceral Leishmaniasis from endemic and non-endemic areas. In this study the most sensitive and the least specific approach was represented by counting the number of skin lesions, which identifiel 99% of MB leprosy patients. ML Flow HSA tests correcty allocated 70.9% and 68.6% of patients in the PB group, and 87% and 81% of patients in the MB group, respectively. The study suggests that the carrier protein of the antigen did not influence significantly in the seropositivity of studied groups. PGL-I serology did not presented cross-reactions with Visceral Leishmaniasis serum samples and confirmed a low positivity among control groups.
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MOURA, R. S. Avaliação de diferentes métodos para a classificação de pacientes e de carreadores de antígenos empregados na sorologia de hanseníase. 2014. 127 f. Tese (Doutorado em Medicina Tropical e Saúde Publica)–Universidade Federal de Goiás, Goiânia, 2014.