Identificação e caracterização de moléculas envolvidas na interação de Paracoccidioides brasiliensis com o hospedeiro
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Data
2009-03-31
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Universidade Federal de Goiás
Resumo
Paracoccidioides brasiliensis causes paracoccidioidomycosis (PCM), a systemic
mycosis presenting clinical manifestations ranging from mild to severe forms. A P.
brasiliensis cDNA expression library was produced and screened with pooled sera from
PCM patients adsorbed against antigens derived from in vitro-grown P. brasiliensis
yeast cells. Sequencing DNA inserts from clones reactive with PCM patients sera
indicated 35 open reading frames presenting homology to genes involved in metabolic
pathways, transport, among other predicted functions. The complete cDNAs encoding
aromatic L-amino acid decarboxylase (Pbddc), lumazine synthase (Pbls) and a
homologue of the high affinity copper transporter (Pbctr3) were obtained. Recombinant
proteins PbDDC and PbLS were obtained; a peptide was synthesized for PbCTR3. The
proteins and the synthetic peptide were recognized by sera of patients with confirmed
PCM and not by sera of healthy patients. Using the vivo-induced antigen technology
(IVIAT) we identified immunogenic proteins expressed at high levels during infection.
Quantitative real - time RT-PCR demonstrated high transcript levels of Pbddc, Pbls and
Pbctr3 in yeast cells infecting macrophages. Transcripts in yeast cells derived from
spleen and liver of infected mice were also measured by qRT-PCR. Our results suggest
a putative role for the immunogenic proteins in the infectious process of P. brasiliensis.
Descrição
Palavras-chave
paracoccidioides brasiliensis , proteínas imunogênicas , processo infeccioso , paracoccidioides brasiliensis , immunogenic proteins , infectious process , 1. Paracoccidioides brasiliensis 2. Proeinas imunogênicas 3.
Processos infecciosos 3. DOPA Descarboxilase 4. Lumazina sintase
4. Transportador de cobre de alta afinidade
Citação
DANTAS, Sabrina Fonseca Ingênito Moreira. Identification and characterization of involved molecules in the interaction of paracoccidioides brasiliensis with the host. 2009. 116 f. Tese (Doutorado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2009.