Avaliação do perfil de subclasses de IgG como fator prognóstico de gravidade de dengue
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2014-09-22
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Universidade Federal de Goiás
Resumo
Variations in IgG subclass concentrations are associated with numerous infectious
diseases. Observations on dengue hemorrhagic fever pathogenesis suggest a high
degree of immune stimulation with extensive activation of complement system preceding
the onset of shock, signaling a possible role of complement activation products in
endothelium damage and plasma leakage. Antibodies IgG1 and IgG3 are known to
activate the complement system efficiently while IgG2 and IgG4 are weak activators.
Moreover, these are the subclasses that exhibit cytophilic properties. Studies have
demonstrated that antibodies in association with FcRs receptors, present on the surface
of numerous cells of the organism, are involved with both, protection and dengue
imunopathogenesis. In the present study, IgG subclass antibodies antigen-specific and
also non DENV specific were determined in dengue seropositive patients through
respectively semi-quantitative and quantitative enzyme immunoassays “in house”
procedures. Serum samples of individuals participating in this study were collected in the
epidemic period of october 2005 to march 2006 on dengue reference health care units in
the city of Goiânia-GO. Study groups were divided as follows: 30 – dengue fever (DF); 30-
dengue with complications (DCC) and 30- dengue hemorrhagic fever (DHF). Overall, it
was observed an increase in total IgG1 and IgG3 in dengue more severe clinical forms,
except in the age group of 25-45 years in which the highest concentration of IgG1 was
observed in subjects with DF and not DCC nor DHF. As for antigen-specific subclasses, it
was found an IgG1 raise and IgG3 decrease in the most severe clinical forms of the
disease. It was also observed a significant difference in IgG1 and IgG3 concentration
between the most severe clinical forms, DCC and DHF. DCC clinical form was
characterized by a larger concentration of IgG3 DENV-specific while the clinical form of
DHF was characterized by an increased amount of total and specific IgG1. The results of
this study indicates that it may be a correlation between IgG subclasses concentrations,
mainly IgG1 and IgG3, and dengue prognosis.
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JUNQUEIRA, I. C. Avaliação do perfil de subclasses de IgG como fator prognóstico de gravidade de dengue. 2014. 122 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2014.