Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae)
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2015-06-30
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Universidade Federal de Goiás
Resumo
The main transmitter species of serotypes of the dengue virus is Aedes
aegypti, the most competent mosquitoes and more adapted to urban and
man-made environment. The control measures of this disease are still mainly
on the vector. Recently, several studies have shown the use of botanical
origin of substances with potential insecticide in control of Ae. aegypti,
alternatively opposite resistance to conventional insecticides. The efficacy
oleoresin of C. langsdorffii the control of Ae. aegypti demonstrated in field
work, signals the promising possibility of this botanical compound to control
this vector requiring studies to clarify the mechanism of action. Thus, it is
proposed to establish the mechanism of death process in the third stage
larvae of Ae. aegypti through morfohistologic studies. The larvae of Ae.
aegypti were subjected to oil-resin solutions and hexane fraction C.
langsdorffii in concentrations of 70 ppm and 80 ppm, respectively.
Subsequently collected in time intervals of 6h, 12h and 24h. They were fixed
in 4% paraformaldhyde in 0.1 M sodium phosphate buffer pH 7.2, embedded
in resin, mounted, stained with hematoxylin-eosin and analyzed by light
microscopy. The oil-resin and hexane fraction C. langsdorffii, caused the
death of the larvae by the destruction of the posterior cells of the midgut
region through cytoplasmatic vacuolation, apical cytoplasmic vesicle
formation, lip brush degeneration, increased cellular volume and fold in
peritrophic matrix. The changes described in this paper demonstrate how
bioactive compounds can trigger cell degeneration and accelerate cell
disruption.
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SANTOS, D. B. Atividade larvicida da Copaifera langsdorffii (Leguminosae), evidenciada pelas alterações morfohistológicas em Aedes aegypti (Diptera, Culicidae). 2015. 71 f. Dissertação (mestrado em Biologia das Interações Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2015.