Variabilidade e estrutura genética espacial em Glossophaga soricina com ocorrência no cerrado
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2013-06-19
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Universidade Federal de Goiás
Resumo
Microsatellite markers are important tools for molecular ecology studies,
particularly for bats, whose information is difficult to obtain through direct
observations. In the first chapter, we conducted searches for scientific articles
about the use of microsatellite markers in bats in order to evaluate the current
knowledge about the genetic patterns and also to unravel sociological aspects
of this knowledge. We found that the use of microsatellite markers to study
bats is quite new and little spread. Many questions in molecular ecology can
be addressed with a limited number of polymorphic markers, such as
microsatellites. This will not only contribute to the knowledge of the species
biology, but also to design effective strategies for conservation of bat species.
In the second chapter, we report the development and characterization
of ten microsatellite loci for the bat Glossophaga soricina isolated from a
shotgun genomic library. Among 67 individuals, the number of alleles per
locus ranged from 2 to 20, and the observed and expected heterozygosity
ranged from 0.015 to 0.606 and from 0.016 to 0.915, respectively. The high
combined probability of genetic identity (4.369x10-8) and probability of
paternity exclusion (0.996) showed that the microsatellite loci are useful for
population genetic structure and detailed parentage studies in natural
populations of G. soricina.
In the third chapter, we used the nine developed microsatellite loci and
spatially explicit analysis to unravel population genetic structure and how
landscape features affected genetic diversity of G. soricina at 17 localities in
the Brazilian Cerrado. Our results showed that G. soricina populations already
have higher inbreeding in fragmented landscapes in small geographic scales.
Also, some pairs of populations showed genetic discontinuity as the outcome
of landscape modification.
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OPREA, Monik. Variabilidade e estrutura genética espacial em Glossophaga soricina com ocorrência no cerrado. 2013. 114 f. Tese (Doutorado em Ecologia e Evolução) - Universidade Federal de Goiás, Goiânia, 2013.