Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus
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Data
2014-04-23
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Universidade Federal de Goiás
Resumo
Staphylococcus saprophyticus is characterized as uropathogenic bacteria that causes
urinary tract infections especially in young women. Some virulence factors were
elucidated, however, little is known about how this bacteria install itself at host human.
The urease was the first virulence factor described in S. saprophyticus, being
responsible by increasing of the bladder pH in infected patients. This is the first study
about S. saprophyticus in proteomics and immunoproteomics perspective of the secreted
proteins (exoproteome) of this bacterium. A total of 44 new secreted proteins were
detected by mass spectrometry. Among the proteins found, five of them have a crucial
role in the glycolytic pathway: Triosephosphate isomerase (TPI), enolase (ENO),
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Glucose-6-phosphate
isomerase (GPI), however, its role as moonlighting proteins have been observed in
various microorganisms. Through of the immunoproteomics, it was possible to detect
18 protein species that reacted onto Western-blotting, and 5 of them could be identified
by mass spectrometry. The most abundant protein specie identified as immunogenic in
S. saprophyticus, it was transglycosilase IsaA (Immunodominant staphylococcal antigen
A), being it well described in Staphylococcus aureus as virulence factor. Another
abundant protein found as immunogenic was Ssa (Staphylococcal secretory antigen),
which, it was identified under 3 isoforms. The enolase was the last protein identified at
immunoproteome of S. saprophyticus. It was possible to conclude from these results
that S. saprophyticus has a wide of extracellular proteins capable to promote bacteria
adaption, and some of them are involved in oxidative/nitrosative stress (SOD and
AhpC), besides possess proteins that have the capacity to incite the humoral immune
response in BalbC mice. All this lead us to hypothesize that S. saprophyticus have
defense and virulence mechanisms in order to protect itself against extracellular stresses
and appropriate mechanisms to promote urinary tract infections.
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OLIVEIRA, L. S. Perfil do exoproteoma e identificação de proteínas imunogênicas secretadas por Staphylococcus saprophyticus. 2014. 81 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2014.