Mastite subclínica: patógenos isolados e respectiva sensibilidade antimicrobiana, variação da contagem de células somáticas e fatores de risco
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Data
2010-02-25
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Universidade Federal de Goiás
Resumo
This study was conducted on 12 dairy farms and in laboratories
Bacteriology and Quality of Milk, both of the School of Veterinary Medicine
Federal University of Goias objective of this study to characterize the
microorganisms in milk from cows with subclinical mastitis and
relate the pathogens identified with the variation of cell count
somatic (CCS), also identify the risk factors and the likely sources of
infection to subclinical mastitis, by visual observations, application of
questionnaires, by isolation and identification of pathogens from the hands of
milkers, swabs and solutions liners and pre posdipping. The profile
sensitivity to key antimicrobial and bactericidal effect of the extract of
Calendula officinalis has been determined on the pathogens isolated in most cases
subclinical mastitis in herds. To evaluate the variation of
CCS in relation to the pathogens involved, we used analysis of frequency and
Chi-square, and the risk factors were analyzed for
Logistic regression to test associations between variables and increased
CCS. It was found that agents with higher frequency of isolation were S.
aureus (28.8% of samples), E. coli (19.8%) and Enterobacter spp. (11.3%). CCS
average herd was approximately 875 x 103cél/mL, and the type
etiologic agent of a significant influence on the variation in SCC.
It was found that S. aureus and Streptococcus spp. were responsible for
greater increase in SCC, with an average of 1192 x 1174 x 103cél/mL and 103cél/mL,
respectively. This variation was significantly higher (p <0.05) when
compared to the average SCC in milk were the other isolates
microorganisms: S. coagulase negative, Pseudomonas spp., E. coli and
Enterobacter spp. It was found that the risk factors that showed
significant association with increased SCC were unsatisfactory hygiene
environment and milker, inadequate drying of the teats, and factors related to
milking equipment, such as poor maintenance and inadequate cleaning. The
hands of the milkers and the sets of liners were able to
convey both the infectious agents as the environment, important in
epidemiology of bovine mastitis. It was also concluded that the extract of marigold
showed bactericidal activity in vitro against S. aureus isolates, and
antimicrobial agents used showed variation in the spectrum of sensitivity.
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Citação
COSTA, Anna Carolina da. Subclinical mastitis: pathogens and their sensitivity
antimicrobial variation of somatic cell count and factors risk. 2010. 102 f. Dissertação (Mestrado em Ciências Agrárias - Veterinaria) - Universidade Federal de Goiás, Goiânia, 2010.