Análise da diversidade genética e mutações no gene da integrase de isolados do HIV-1 de pacientes atendidos no município de Jataí/Goiás
Nenhuma Miniatura disponível
Data
2018-04-11
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
INI have been available in Brazil since 2009, when the first INI, Raltegravir, became available for
therapy of rescue of HIV + patients in therapeutic failure. In the year 2017, a second INI was
introduced into ART of patients newly diagnosed with HIV-1, Dolutegravir, which had a higher
genetic barrier and a single daily dose, replaced Efavirenz in the first line of treatment. However,
despite the efficiency of INI, the emergence of viral variants resistant to these drugs is inevitable.
For this reason, it is necessary to monitor resistance mutations to INI, which may lead to therapeutic
failure, aiming at optimizing the therapeutic regimen and controlling HIV infection. The objective
of this study was to evaluate the occurrence of INI mutations and the resistance profile in HIV + /
AIDS patients in the city of Jataí/Goiás. The complete IN gene was sequenced from samples from
INI-naive patients. Resistance mutations were identified by the Stanford-HIV and IAS-USA
database. Viral subtypes were identified by phylogenetic analysis. Among the 52 samples analyzed,
no primary mutation was identified. Two accessory mutations (T97A / G163K) were identified and
these induce a low level of INI resistance. In total, 152 polymorphisms were identified. The most
prevalent subtype was subtype B. Therefore, these data demonstrate that the IN region is still highly
conserved, encouraging the use of INI in HIV-1 therapy, and assist in the mapping of HIV-1 genetic
diversity in the Southwest region of Goiás.
Descrição
Palavras-chave
Citação
PAULA, M. S. Análise da diversidade genética e mutações no gene da integrase de isolados do HIV-1 de pacientes atendidos no município de Jataí/Goiás. 2018. 94 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2018.