Análise proteômica da fase leveduriforme do fungo patogênico Paracoccidioides sp durante a privação de nitrogênio
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Data
2014-03-13
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Universidade Federal de Goiás
Resumo
The fungi of the genus Paracoccidioides sp are etiologic agents of disease
paracoccidioidomycosis (PCM), are termodimórficos and have the ability to move the
saprobiótica form to yeast form at a temperature around 37 ° C. The uptake is essential
for the growth and adaptation of the fungus in host tissue, where nitrogen-dependent
pathways have close relationship with pathogenicity. Pathogenic organisms possess a
regulatory system called Nitrogen Catabolic Represion which is induced when nitrogen
availability is limiting in surround causing the expression of genes necessary for
nutrient uptake when preferred sources such as glutamine and ammonia are scarce. This
regulatory process comprises a complex system in the infectious process. This study
aims to identify proteins regulated by nitrogen depletion, where the pathogenic fungus
Pb01-like was analyzed as a proteomic response. Pb01-like was grown in minimal
medium control MMcM (+N) and treated MMcM (-N) for 6 hours at 37 ° C,
cytoplasmic proteins were extracted and subjected to tryptic digestion and
quantification. The proteomic profile revealed an expression of 135 proteins, 40
proteins induced or identified the treaty, 58 repressed or identified control and 44
proteins were expressed constitutively. In silico analyzes showed that the gene is
regulated by formamidase areA transcription factor that responds conditions of nitrogen
depletion in Aspergillus nidulans and the surrounding medium the enzymatic activity
for the gene of formamidase Pb01-like induced depletion of nitrogen during. The amino
acid sequence of the regulatory gene areA important in the metabolism of nitrogen was
aligned between Pb01-like, Pb18, Aspergillus nidulans and Neurospora crasssa where
this alignment showed high identity and homology of conserved zinc finger domains
and DUF 1752. Metabolic pathways such as fermentation, gluconeogenesis, protein
synthesis, nitrogen metabolism were induced for the depletion of nitrogen, showing a
possible modulation of the metabolism of the fungus in order to be able to adapt to
environments with nitrogen limitation.
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CRUZ-LEITE, Vanessa Rafaela Milhomem. Análise proteômica da fase leveduriforme do fungo patogênico Paracoccidioides sp durante a privação de nitrogênio. 2014. 90 f. Dissertação (Mestrado em Genética e Biologia Molecular) - Universidade Federal de Goiás, Goiânia, 2014.