Variabilidade genética e avaliação de sensibilidade a fungicidas em sclerotinia sclerotiorum proveniente de cultivo irrigado de feijoeiro

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Universidade Federal de Goiás


Sclerotinia sclerotiorum is a phytopathogenic fungus that infects more than 400 plant species, including common bean. Genetic variability studies in connection with phenotypic traits of agronomic interest are important to drive the control strategies against this pathogen. The aims of this study were: to evaluate the genetic variability, fungicide sensitivity, aggressiveness and to determine the proportion of MAT (Mating Type) alleles of 79 isolates of S. sclerotiorum distributed in four populations from common bean. Two populations represented a single sampling location in two different times (2000 and 2013 growing seasons). To evaluate the fungicide sensitivity a cell viability test based on the alamarBlue dye using mycelial growth was standardized. Dose-response curves for fluazinam, procymidone and benomyl were estimated using this test and were compared with dose-response curves estimated by the mycelial growth inhibition on PDA plate and the FRAC (Fungicide Resistance Action Committee) protocol. Despite the differences to assess the fungicide sensitivity between the three methods, the dose-response curves showed similar trends for the three fungicides. The fungicide sensitivity assessment at the four populations showed low sensitivity to benomyl in the Planaltina population. Furthermore, this population presented a principally clonal population structure, with a haplotype represented by 18 out of 20 isolates. Significant population differentiation in all pairwise comparisons of phi, except the comparison between EV_2013-NH, was detected. Five genetically homogeneous groups were inferred by the DAPC analysis. No group was conformed by isolates from the four populations. Only two haplotypes between the two populations from the same sampling location were shared. The hypothesis of random mating was rejected at the four populations; however this hypothesis was not rejected at the two major populations inferred by the DAPC analysis. The screening of mating type locus showed a dominance of Inv+ isolates and a high proportion of Inv+/Inv- isolates (presumable heterokaryons).



ARBOLEDA, William Andrés López. Variabilidade genética e avaliação de sensibilidade a fungicidas em sclerotinia sclerotiorum proveniente de cultivo irrigado de feijoeiro. 2015. 79 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Goiás, Goiânia, 2015.