Avaliação do efeito do bevacizumab, 5-fluoruracila e triancinolona na modulação cicatricial de feridas cirúrgicas em ratos
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2017-03-13
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Universidade Federal de Goiás
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The aim of the present study was to analyze the influence of 5-Fluorouracil (5Fu), Triamcinolone (T) and Bevacizumab (BVZ) on scar modulation in an experimental model of surgical lesions performed on rats. Rattus novergicus albinus rats were selected, divided into four groups: Group 1: BVZ; Group 2: 5Fu + T; Group 3: BVZ + 5Fu + T and Control Group (CG): received no medication. The incision surgery performed was linear, wedged and sutured in the first intention, similar to the surgical incision of upper blepharoplasty, followed by suturing the edges with simple stitches using 6.0 nylon thread. Stitches were removed on the 7th postoperative day. Immediately after, the medications from each group were applied by subcutaneous retro injection below the surgical scar. Euthanasia and tissue removal, which contained the surgical scar and 2 mm margin, were performed on the 14th day of the experiment. In the macroscopic evaluation, performed daily, no delay, suture dehiscence or signs of infection were observed. For histological evaluation, the tissue was stained with hematoxylin eosin and picrosirius. In the morphometric analysis between groups, there was difference in the number of monocytes only in group 2 (p = 0.03). There was a 56%, 86% and 85% decrease in the number of neovasculars in groups 1, 2 and 3 respectively (p = 0.002). There was no statistical difference in the number of fibroblasts, hemorrhage and hemosiderin. Analysis of picrosirius staining by electron microscopy showed an increase in collagen density (Group 1: 16%, Group 2: 87% and Group 3: 59%) and a more organized disposition in the groups receiving the medications compared to CG (p <0.001). Scar modulation was observed in all groups, and group 2 achieved better results. This was the first experimental study to evaluate the influence of the three medications together on healing. Drugs when used together can act in the prevention and treatment of unsightly scars, and these associations act in different ways of healing and have promising alternatives of molecular action as new options to the current treatment that is based on the use of corticosteroids that in addition to not responding in some patients, lead to unwanted and frequent side effects in their use alone.
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RIOS, J. E. S. Avaliação do efeito do bevacizumab, 5-fluoruracila e triancinolona na modulação cicatricial de feridas cirúrgicas em ratos. 2017. 70 f. Dissertação (Mestrado em Ciências da Saúde) - Universidade Federal de Goiás, Goiânia, 2017.