PERFIL SOROLÓGICO EXPERIMENTAL DE CAMUNDONGOS INFECTADOS COM A CEPA CISTOGÊNICA ME-49, DE Toxoplasma gondii ANTES E PÓSTERAPÊUTICA ESPECIFICA.
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2008-04-01
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Universidade Federal de Goiás
Resumo
Toxoplasmosis is a protozoosis of high incidence in the world, caused by
Toxoplasma gondii, transmitted by the ingestion of food contaminated by oocysts
found in feline feces, or cists in raw or underdone meat, congenitally in other
instances. The disease is usually asymptomatic, but in embryos or in
imunodrepessive patients it may be devastating. In this work, the experimental
serologic profile has been evaluated after specific therapy in isogenies mice Balbc,
infected with a cystogenic lineage ME-49 of T. gondii. Fifty animals have been
intra-peritoneal inoculated with 10 cists of T. gondii (lineage ME-49) obtained from
the maceration of mice brains of previously inoculated mice. The mice have been
divided into three groups of 15 animals each and a negative control group (not
infected and not treated) of 5 animals. All the animals have been daily
accompanied, and the symptoms were verified during 20 days. After this period,
two groups of 15 animals underwent specific therapy schemes in which; group A:
pirimetamin (12,5mg/kg/day) + folic acid; group B: pirimetamin + folic acid +
sulfadiazine (500mg/kg/day), orally administrated during 10 consecutive days. After
this period, all animals have been scarified, with an interval of 5 days, and the
blood has been collected by a heart puncture and the serum separated for the
Indirect Immuno-fluorescence Reaction. The samples from the treated animals
presented a significant diminution in the quantity of (IgG) antibodies evaluated by
the two schemes by the indirect immuno-fluorescence reaction (t=3,5, and t=7,6
respectively), in relation to the control group. There have been no significant
differences between the two therapeutic schemes (t=0,36). Our results have shown
that the experimental precocious treatment reduces significantly the IgG levels,
suggesting a diminution of antigenic stimulus, and consequently a better prognosis
for toxoplasmosis.
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GUIMARÃES, Liliane Rego. EXPERIMENTAL SOROLÓGICO PROFILE
OF MICE INFECTED WITH
Cystogenic ME-49, DE
Toxoplasma gondii BEFORE AND AFTER THERAPY
SPECIFICATIONS.. 2008. 98 f. Dissertação (Mestrado em Medicina) - Universidade Federal de Goiás, Goiânia, 2008.