Caracterização proteômica da fração nuclear da forma leveduriforme de espécies de Paracoccidioides
Carregando...
Data
2014-08-01
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Paracoccidioidomycosis (PCM) is an endemic disease in Latin America caused by fungi of
the genus Paracoccidioides which is composed of two species P. lutzii e P. brasiliensis.
The fungus grows as mycelium in saprophytic environment, and as yeast in host.
Paracoccidioides spp present multinucleated yeast cells. The characterization of the
nuclear proteome an important step to understand the biology of the fungus, and
contributes to future studies of possible pharmacological targets. In this study we aimed to
characterize the profile of the nuclear proteome of Paracoccidioides species in yeast form.
A total of 570 proteins were identified and classified as nuclear, using a combination of
method sample enrichment, proteomic of high accuracy technique, NanoUPLC-MS
E
, and
bioinformatic filters. The results revealed important proteins related to DNA maintenance,
regulation and gene expression, synthesis and processing of messenger and ribosomal
RNA and nuclear-cytoplasmic traffic. Uncharacterized proteins and proteins commonly
known as cytoplasmic were identified in fraction enriched nuclear and require further
studies to demonstrate its nuclear roles. This is the first descriptive nuclear proteome of
Paracoccidioides spp. and opens the way for new researches.
Descrição
Palavras-chave
Citação
OLIVEIRA, Lucas Nojosa. Caracterização proteômica da fração nuclear da forma leveduriforme de espécies de Paracoccidioides. 2014. 102 f. Dissertação (Mestrado em Biologia das Relações Parasito-Hospedeiro)–Universidade Federal de Goiás, Goiânia, 2014.