Caracterização histológica de vermes adultos machos de Lagochilascaris minor
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Data
2012-02-27
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Universidade Federal de Goiás
Resumo
Nematoda Lagochilascaris minor is the etiologic agent of lagochilascaríase in
human beings. Until today, no one knows the natural host of this helminth and Brazil
registers about 90% of the cases of lagochilascaríase world-wide human being. The life
cycle of L. minor was described using an experimental model in mice and domestic cat,
behaving as an intermediate host and definitive host respectively. The objective of this
study was to evaluate the histology of the adult males of this helminth worms, using light
microscopy. These specimens were removed after necropsy of cats kept in their cages
cataloged in male and female worms, and the adult male specimens were fixed in formalin
solution 10 % hot, AFA and 4% paraformaldehyde in sodium cacodylate buffer 0 1 M, pH
7.2. After the different fixations, the samples were dehydrated in a gradual series of
ethanol in different concentrations of alcohol levels, and included in historesin cut with
3μm –thick microtome, with horizontal and transverse serial sections. The sections were
stained with Hematoxylin & Eosin (HE), Carmin, Giemsa and Toluidine Blue. The best
fixation was achieved with the use of AFA and 4% paraformaldehyde in 0.1 M sodium
cacodylate buffer pH 7.2 and the best staining was obtained from the use of the dye HE,
which showed histological structures of adult male worms L. minor loop as testicular,
seminal vesicle, ejaculatory duct, sperm and spicule. It was also observed structures of the
digestive system, muscular system, cuticle, wing side and lateral line. The sections were
photomicrographed using photomicroscope with the help of the program Motic Images Plus
2.0, selected and analyzed.
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VIEIRA, Ana Paula de Toledo. Caracterização histológica de vermes adultos machos de Lagochilascaris minor. 2012. 66 f. Dissertação (Mestrado em Medicina Tropical e Saúde Pública) - Universidade Federal de Goiás, Goiânia, 2012.