Efeito de Metarhizium anisopliae s.l. combinado com extratos hidroalcoólicos de plantas em Aedes aegypti
Nenhuma Miniatura disponível
Data
2017-02-09
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
Entomopathogenic fungi combined with plant extracts may have their development in vitro and their insecticidal activity modified. There are many reports on the activity of entomopathogenic fungi and extracts in insects, but few studies on fungi combined with plant extracts. The objective of this study was to evaluate in vitro the effect of crude ethanol extracts of Artemisia annua, Andrographis paniculata, Curcuma zedoaria, Ginkgo biloba and Rosmarinus officinalis on the Metarhizium anisopliae s.l. IP 46 by the diffusion disc test, to evaluate in vivo susceptibility of Aedes aegypti larvae to extracts by determination the cumulative emergence of adults, development and mortality of larvae for 15 days, then to select the extracts and combine them with IP 46 at 3.3 x 105 conidia.mL-1. There was no significant inhibition germination of IP 46. On the fifteenth day there was a significant effect on cumulative emergence of adults and development of larvae for A. annua at 10 ppm, 33 ppm and 1000 ppm and C. zedoaria at 100 ppm and 333 ppm. There was also a significant effect on accumulated mortality of larvae exposed to G. biloba at 10000 ppm.These extracts with in these concentrations were selected and combined with IP 46 at 3.3 x 105 conidia.mL-1, minus G. biloba which was combined at 5000 ppm after the LC50 and LC90 calculations. These combinations, except for A. annua at 1000 ppm, exhibited a significant increase in larval mortality and/ or showed an effect on development, indicating that combined methods may be more effective in controlling A. aegypti than isolated methods.
Descrição
Palavras-chave
Citação
SILVA, D. C. Efeito de Metarhizium anisopliae s.l. combinado com extratos hidroalcoólicos de plantas em Aedes aegypti. 2017. 60 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2017.