An€álise da resposta de Paracoccidioides brasiliensis a diferentes tipos de agentes estressores e osmoreguladores e express o heteró‚loga e localizaçムo de β-1.3-glicana sintase
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2010-02-26
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Universidade Federal de Goiás
Resumo
Paracoccidioides brasiliensis is a thermo-dimorphic human pathogenic fungus
that lives at 23”C in the mycelium phase (infecting phase) and at 37”C in the yeast
phase (parasite phase). In attempt to survive, the cell wall of fungi can change its
composition and/or structure in response to environmental stress by compensatory
mechanisms. The molecules involved in these mechanisms are possible target for the
development of effective antifungal agents. In P. brasiliensis, the main components of
the cell wall are glucans and chitin polymers. These polymers make a primary barrier
that is responsible for the structural integrity and form of the cell wall. In this work the
behavior of P. brasiliensis was evaluated against stress conditions with the aim of study,
for the first time, the mechanisms used by this fungus in the maintenance of the cell
wall integrity. Our results shown that P. brasiliensis yeast cells are sensitive to cell wall
stressors calcofluor white (CFW), congo red (CR), SDS, KCl, NaCl and sorbitol. There
was an increase in the PbFKS1 transcripts expression and in the content of cell wall β-
1,3-glicana after treatment with all stressor agents. After treatment with SDS and KCl
the PbGFA1 transcripts expression and the cell wall GlcNAc residues also increased.
The transcript expression of PbGEL3 was also evaluated being increased after treatment
with CFW, NaCl and sorbitol. Thus we showed that these molecules are involved in the
maintenance of the cell wall against stress conditions. Apart from these analyses we
obtained the active recombinant protein PbFks1pc. Through the anti-PbFks1pc antibody
we performed immunocitolocalization assays. These experiments revealed the
localization of PbFks1p in regions of apical growth in the mycelium phase and in the
cell surface in yeast phase.
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TOMAZETT, Patrícia Kott. Analise response of Paracoccidioides brasiliensis to different types of stress agents and osmoreguladores and expresses Heteralogs and Location of the β-1.3-glucan synthase. 2010. 125 f. Tese (Doutorado em Ciencias da Saude) - Universidade Federal de Goiás, Goiânia, 2010.