Detecção do DNA de Chlamydia trachomatis em espondiloartopatias e artrite reumatóide
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2004-04-20
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Universidade Federal de Goiás
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Introduction: Chlamydia trachomatis is the bacteria responsible for the most
prevalent sexually transmitted disease worldwide. Most of the infections in men
and women is asymptomatic and when undiagnosed and untreated may reach the
joints causing not only arthritis, but also other acknowledged complications related
to the female reproductive system.
Objective: To investigate C. trachomatis DNA in the urine and synovial fluid from
patients with spondyloarthropathies (SpA) and rheumatoid arthritis (RA) and
evaluate serum anti-C. trachomatisIgG and IgM antibodies.
Methods: The population consisted of 15 patients with spondyloarthropathies,
being nine with undifferentiated spondyloarthropathy (US) and six with reactive
arthritis (ReA) (group I), and 15 patients with rheumatoid arthritis (RA) (group
II). The chlamydial DNA was assessed in synovial fluid and urine samples of all
patients by Amplicor PCR. The anti-chlamydial IgG and IgM antibodies were
quantified through indirect imunofluorescence (IIF), while 15 patients of group I
were typed for HLA-B27 by the use of flow citometry. Social demographical data
and all information on sexual behavior and presence of symptoms were collected
through a (questionnaire in the form of) an interview.
Results: C. trachomatis DNA was found in only one synovial fluid sample from
patient with ReA (6,7%). In two patients with RA, chlamydial DNA was identified
in the urine sample (13,3%). The anti-chlamydial IgG antibodies were present in
eight patients of the population studied; being three patients from group I (20%),
and five from group II (33,3%). The greatest titer of this antibody 1/256 was
associated with the presence of chlamydial DNA in a patient from group II. The
IgM antibody was not detected in any of the samples from both groups. Four
individuals from group II (26,7%) were HLA-B27 positive and its presence was
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related to sacroiliitis.
Conclusion: The results in this study show that in patients with
spondyloarthropathies and rheumatoid arthritis, presenting a picture of articular
activity one might not exclude C. trachomatisas the triggering agent.
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FERNANDEZ, Rafael Navarrete, Detecção do DNA de Chlamydia trachomatis em espondiloartopatias e artrite reumatóide. 2004. 86 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2004.