Criopreservação de ápices caulinares e micropropagação em condições heterotróficas e mixotróficas de eugenia dysenterica (Mart.) DC.
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2015-02-23
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Universidade Federal de Goiás
Resumo
Conventional micropropagation systems often use culture media supplemented with
sucrose and sealed bottles. This system is called heterotófico as exogenous carbohydrates
are the only source of energy for the plant. Mixotróficos systems, although still adding
sucrose in half, have the differential to allow gas exchange between the internal and
external environment of the culture flask. This ventilation brings many benefits to plant
growth in vitro. By the constant loss of the Savannah area, it is necessary to improve
micropropagation protocols and germplasm conservation of the species within it.
Cryopreservation is considered the best for long-term preservation method and the
encapsulation-dehydration technique have been promising for tropical species. The
objectives were to compare the heterotrophic system in vitro propagation of Eugenia
dysenterica (Mart.) DC. mixotrófico with a system, and get a method of germplasm storage
dysenterica E. vitro by cryopreservation. Identified Gems' positions in the stem (closer to
the root or the apex) were inoculated in tubes with or without gas exchange in WPM
medium supplemented with 0,00μΜ, 0,54μM, 2,69μM, and 5,37μM, NAA, and 0,00μM,
4,44μM, 11,10μM and 17,76μM BAP in all possible combinations. For rooting was used
WPM medium supplemented with IBA to 0,00μM, 9,84μM, 19,68μM, and 29,52μM. The
system of gas exchange was greater in the number of sheets in most analyzed variables.
The type of gem had little influence on the variables. The best treatment in heterotrophic
and mixotrófico systems was 29.52 mM of IBA, with 33.33% and 16.66% respectively
rooting. Acclimatization was performed successfully in both systems. For cryopreservation
was used apexes obtained from plants grown in vitro. The apices were initially suspended
in MS medium supplemented with 3% solution of sodium alginate, 0.4 M glycerol and
4,44μM BAP and dispensed in MS medium solution (free of calcium) supplemented with
0.1 M calcium chloride, 0.4 M glycerol and 4,44μM BAP. The summits were held in three
exposure levels of sucrose (0.25M, 0.5M, and 0.75M) combined will three levels of
desiccation (0 h, 1 h, 2 h) before being frozen in liquid nitrogen. There was no regeneration
in cryopreserved apexes. The best treatment for non-cryopreserved apices consisted of
0.25M sucrose and 1 h of drying. The null regeneration of cryopreserved apices which
indicates that the stress is related to desiccation stress freezing.
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SILVEIRA, A. A. C. Criopreservação de ápices caulinares e micropropagação em condições heterotróficas e mixotróficas de eugenia dysenterica (Mart.) DC. 2015. 74 f. Dissertação (Mestrado em Genetica e Melhoramentode Plantas) - Universidade Federal de Goiás, Goiânia, 2015.