Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel
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2015-03-30
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Universidade Federal de Goiás
Resumo
The honey is a concentrated source of nutrients, it is used by animals and
exploited as a natural resource for homes since antiquity. Many studies have tried to
characterize the honey and its quality, but the complex natural process of production,
involve that any research is not able to obtain fully satisfactory results. In this work 24
samples. Were studied 24 samples from honey producing regions located around
Goiania - GO and some foreign samples (Portugual, USA and New Zelândias), those
donated by the Beekeeping Association of the State of Goiás and these purchased in
specialty stores in their respective countries . The physical and chemical tests as
indicative of deterioration, hydroxymethylfurfural (HMF) and diastase activity (AD),
followed parameters proposed by Normative Instruction N. 11/2000 of the Ministry of
Agriculture, Livestock and Supply (MAPA). The antioxidant components analysis was
used for traditional spectroscopic methods Folin-Ciocalteu (FC), Ferric Reducing
Antioxidant Potential (FRAP), 2,2-diphenyl-1-picryl-hydrazyl (DPPH●); and
electroanalytical calculated from the oxidation peaks voltammetric differential pulse
(DPV) by Electrochemical Index (EI). While mid-infrared method (IR) and methods of
multivariate statistical analysis were used for identification and classification of honey
from different floral sources and/or regions. As for the deterioration analysis test
(HMF and AD) 50% of the samples applied in these tests, for the control of physical
and chemical quality, were at odds with the Normative Instruction N. 11/2000 of
MAPA. All samples showed the presence of antioxidant compounds exposed by
methods FC, FRAP, DPPH● and EI; and the method of FRAP was the one closest to
the EI and the DPPH● was inversely proportional to EI, which may conclude that the
electroanalytical by DPV is suitable for analysis of antioxidant compounds in honey.
The infrared results coupled with chemometric tool, principal component analysis
(PCA) and hierarchical cluster analysis (HCA), were not satisfactory for sample
groups of the same floral origin and/or region, but suggested trend in results.
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Citação
REZENDE, S. G. Métodos eletroanalíticos e ensaios de atividade antioxidante no controle de qualidade de mel. 2015. 53 f. Dissertação (Mestrado em Ciências Farmacêuticas) - Universidade Federal de Goiás, Goiânia, 2015.