Desenvolvimento de filmes bioativos contendo probiótico (Lacticaseibacillus rhamnousus GG.) e polpa de mirtilo (Vaccinium myrtillus)

Abstract

The growing demand for functional foods, driven by the pursuit of healthier eating habits, has brought attention to ingredients such as blueberry (Vaccinium myrtillus), a fruit rich in bioactive compounds like anthocyanins, which exhibit remarkable antioxidant and therapeutic properties. In this context, the incorporation of active components into edible films has been widely studied as an effective and practical method for delivering these substances in a stable form. Aligned with this trend, the use of probiotics in such films aims not only to ensure their viability but also to add nutritional value. This study aimed to develop bioactive films based on carboxymethylcellulose (CMC), the probiotic Lacticaseibacillus rhamnosus GG, and freezedried blueberry pulp. The purpose of these films is to act as carriers for the delivery of functional compounds. To assess their effectiveness, the films were characterized in terms of morphology, moisture content, solubility, water vapor permeability, thickness, vitamin C content, anthocyanins, color, and mechanical properties. In addition, the viability of the probiotic was analyzed over 45 days at storage temperatures of 4°C and 25°C. The incorporation of blueberry pulp influenced not only the physical and optical characteristics but also endowed the films with bioactive properties, such as increased vitamin C content. A reduction in water solubility and an increase in film thickness were observed features that are desirable for protective barrier applications. However, a decrease in mechanical strength and an increase in water vapor permeability were also noted. These changes may be related to the irregular structure observed in the micrographs, suggesting greater diffusivity of water molecules. The addition of the probiotic L. rhamnosus GG represented a functional advantage for the films, with the potential to contribute to human health benefits.