Atividade de compostos naturais e sintéticos na presença de espécies de Candida
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2017-02-09
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Universidade Federal de Goiás
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Infections caused by yeasts of the genus Candida are called candidiasis and are considered
opportunistic diseases with characteristics ranging from superficial to disseminate. Candida
species may develop defense mechanisms to the major antifungal agents available for clinical
use against infections, making it difficult and limiting treatment. Natural products can be a
source of bioactive compounds against infectious diseases, such as the Curcuma longa Linn or
saffron plant as it is popularly known that it has the compound curcumin. This vegetable is
used in large scale by the population as food condiment and as natural medicine against
inflammatory diseases and infectious diseases. Laboratory-synthesized compounds have been
to interest in medical biotechnology, among which the hydrazone derivatives are associated with antimicrobial including antifungal, antioxidant, antiparasitic, anticonvulsive, analgesic,
anti-inflammatory and antitumor activity. The objectives were to determine the minimum
inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of curcumin and
n-acylhydrazone compounds, and also to verify the time-kill curve of Candida, to quantify
ergosterol and to evaluate hemolysis of red blood cells after action of both compounds.
Twenty isolates Candida spp. in the broth microdilution test for evaluation of the MIC and MFC
of the compounds. The time-kill curve and ergosterol assay were checked after treatment of
C. albicans ATCC 28367 with the compounds. To evaluate the cytotoxicity of these
compounds sheep red blood cells were used. The compound curcumin showed MIC values
between 16 and 256 μg/mL and MFC between 128 and values greater than 512 μg/mL. For
the n-acylhydrazone derivative the MIC values ranged from 2 to 128 μg/mL and the MFC
variation ranged from 256 to values greater than 512 μg/mL. The compounds showed
fungistatic action on the growth kinetics of Candida over 48 hours. The determination of
ergosterol after contact with the agents in MIC of 64 μg/mL of curcumin and 16 μg/mL of nacylhydrazone
was reduced by 29.56 and 53.57%, respectively. The compounds showed
hemolytic activity at concentrations of 512 μg/mL. The results observed in this work show
that the curcumin and the n- acylhydrazone derivative have promising antifungal properties.
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ANDRADE, F. A. Atividade de compostos naturais e sintéticos na presença de espécies de Candida. 2017. 61 f. Dissertação (Mestrado em Biologia da Relação Parasito-Hospedeiro) - Universidade Federal de Goiás, Goiânia, 2017.