Avaliação de SNPs associados à descoloração de feijões especiais destinados à exportação e consumo interno
Nenhuma Miniatura disponível
Data
2021-04-29
Autores
Título da Revista
ISSN da Revista
Título de Volume
Editor
Universidade Federal de Goiás
Resumo
The common bean has grains of different colors, shapes and sizes. There are special beans, with large, colorful beans. These beans may show color loss during thermal processing, an important problem for industrialization and commercialization. It has not yet been possible to elucidate the variation in sensitivity to thermal processing during industrialization, which causes color loss in the cooked grain and depreciates its physical quality. Thus, the objective of the research was to characterize and validate SNPs (Single Nucleotide Polymorphisms) markers for the discoloration characteristic in special bean varieties. For the sequencing and evaluation of the SNPs, 21 bean genotypes were used first, eight of Andean origin and 13 of Mesoamerican origin. Then another 20 genotypes of Andean origin were planted, thus totaling 41 genotypes (28 Andean and 13 Mesoamerican) in the final analysis. The genotypes were evaluated for polymorphism of SNPs and fading of post-cooking grains, aiming to expand the base of evaluated genetic materials. Thus, seeds of 41 special bean genotypes were sown in a greenhouse for multiplication. Young trifoliate leaves were collected for DNA extraction, which were used for the sequencing and alignment of the 21 genotypes, in order to find the SNPs associated with grain color loss. Seven regions were sequenced containing target SNPs, previously identified in the literature as associated with discoloration in Andean black bean germplasm. The grains were evaluated for the color difference parameter (ΔE) before and after cooking. by a method similar to the industrial process for canning pre-cooked grain. The results of the sequencing revealed four SNPs for the seven regions evaluated. The ΔE values varied between 4.23 and 22.49 for Andean beans (lowest value for Montcalm), and from 5.63 (BRS FP 403) to 9.35 (BRS Campeiro) for Mesoamericans. Only for Andean beans, associations of the SNP with the lowest values of ∆Ε were identified. For these SNPs, TaqMan probes were developed, which were evaluated with an extended sampling of beans characterized in terms of ∆Ε. Two SNPs were identified with great potential for indirect selection of bean genotypes associated with lower values of ∆Ε, confirmed by simple linear regression (p-value ≤ 0.01). These SNPs will be incorporated into the marker-assisted selection routine within the bean breeding program for the selection of Andean genotypes with less grain discoloration in industrial thermal processing.
Descrição
Citação
SOUSA, A. R. Avaliação de SNPs associados à descoloração de feijões especiais destinados à exportação e consumo interno. 2021. 81 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Goiás, Goiânia, 2021.