Seleção de bactérias da rizosfera de lactuca sativa capazes de bioconverter glicerol em 1,3-propanodiol e 2,3-butanodiol
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2016-05-23
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Universidade Federal de Goiás
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The coproducts production is one of the main setbacks in the biodiesel’s productive chain. One of these coproducts, the most representative is the glycerol. In this aspect, this study aim to contextualize the use and production of fossil fuels and biofuels, mostly biodiesel, and to investigate the biotechnological potencial of lettuce’s (Lactuca sativa) rhizobacteria on glycerol bioconversion into value-added products. For such purpose, it was conducted a literature search about the use of fossil fuels and the biofuels market development, besides the biotechnological potential of the use of glycerol in fermentative processes. From rhizosphere soil samples, it was bioprospected micro-organisms with the ability to utilize glycerol as the sole carbon source and in bioconvert 2,3-butanediol and 1,3-propanediol. In order to preserve the maintenance and development of urban activities a change in the energy matrix is necessary since fossil fuels are a finite resource and shown to be unsustainable or cause impacts in environment and human health. Through the implementation of policies that aimed to invest in research and production of biodiesel Brazil stands out as a major producer of the compound. In the experimental segment, among the sampled and identified microorganisms, the family Enterobacteriaceae was highlighted. All isolated presented biochemical / physiological profile heterogeneous and belonging to genera notedly used in the glycerol bioconversion. Three samples were identified as simultaneous producers of compounds of interest, simultaneously AG3 was the best producer of 2,3-BD and 1,3-PD, 0522 g.L-1 and 0.735 g.L-1, respectively. AG6 stood out in the production of 1,3-PD, 0842 g.L-1. Therefore, the sustainability provided by biofuels, highlighting biodiesel, brings a promising perspective to future and the use of co-products generated by the biodiesel production chain can contribute to the appreciation of biodiesel. Lactuca sativa’s rhizobacteria have the biotechnological potential for bioconversion of glycerol, promising discovery for the development of biodiesel and biorefinery sector.
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SOARES, R. S. Seleção de bactérias da rizosfera de lactuca sativa capazes de bioconverter glicerol em 1,3-propanodiol e 2,3-butanodiol. 2016. 95 f. Dissertação (Mestrado em Ciências Ambientais) - Universidade Federal de Goiás, Goiânia, 2016.