Detecção e genotipagem do papilomavirus humano em amostra do colo do útero de adolescentes do Distrito Sanitário Noroeste do município de Goiânia, Goiás
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2005-03-31
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Universidade Federal de Goiás
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Background: Human Papillomavirus (HPV) infection is a strong predictor for cervical cancer
development. Some HPV types are considered at high-risk and associated with pre-malignant
lesions and cervical cancer. Genital HPV infection is highly prevalent in sexually active young
women. Actually PGMY09/11 primer system associated with Restriction Fragment Length
Polymorphism (RFLP), hibridization or DNA sequencing is one of the most sensitive methods
for detecting and typing HPV-DNA in genital samples.
Objectives: To detect and identify HPV-DNA genotypes in genital specimens among sexually
active female adolescents from Distrito Sanitário Noroeste, Goiânia.
Methods: We performed a cross-sectional study among 432 sexually active female
adolescents (15-19 years), randomly selected at Distrito Sanitário Noroeste, Goiânia, and
served by the Family Health Program. HPV-DNA was detected by PCR assay, in endocervical
samples, using the PGMY09/11 consensus primer set. The presence of amplifiable DNA was
assessed with -globin primers (PCO3/PCO4). Amplicons generated with PGMY primers were
typed with the line blot assay (PGMY-line blot) and RFLP.
Results: HPV-DNA was detected in 121 samples yielding a 28% prevalence (95% CI 23.8-
32.5). All samples yielded a -globin amplimer, confirming the DNA adequacy. Thirty different
HPV genotypes were identified, the high risk genotypes frequently found being: HPV-16
(6,7%); followed by HPV-51 (5,1%); HPV-31 (4,6%); HPV-52 (4,2%) and HPV-18 (3,5%).
PGMY-line blot test identified 54 cases of infection caused by multiple genotypes. The
genotypes 16, 51, 18, 53 and 52 were the most frequently associated with cervical co-
infection with multiple HPV types.
Conclusions: A high prevalence of HPV-DNA was found and a broad spectrum of HPV
genotypes was identified, predominantly high risk HPV genotypes. A high frequency of
multiple HPV infections was found in the studied population.
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DAUD, L. E. S. Detecção e genotipagem do papilomavirus humano em amostra do colo do útero de adolescentes do Distrito Sanitário Noroeste do município de Goiânia, Goiás. 2005. 60 f. Dissertação (Mestrado em Medicina Tropical e Saúde Publica) - Universidade Federal de Goiás, Goiânia, 2005.