Production of lignocellulosic enzymes by Streptomyces thermocerradoensis I3 in unconventional culture medium

Abstract

The present work aimed to contribute with new data about the production of lignocellulolytic enzymes produced by Streptomycesthermocerradoensis I3, isolated from Brazilian central savannah soil (also known as Cerrado), using the rhizome of two plants from the Cerrado, Cochlospermumregium (CR) and Jatropha elliptica (JE), as well as wheat bran (WB) as a culture medium. In addition, verify the influence of pH on the enzymes production using WB and evaluate the effectiveness of enzymatic extract produced in CR and JE flours. The higher CMCase activity detected occur in WB (6.07 ± 0.13 IU mL- 1) followed by JE (4.18 ± 0.23 IU mL-1), while it was not detected in CR. The higher xylanase activity detected was 4.14 ± 0.10 IU mL-1 in WB, followed by 2.13 ± 0.14 IU mL-1 in CR and was not detected in JE. Regarding the influence of pH on the activity of CMCase in WB, was observed that the highest activity occurred at pH 7.5 (13.22 ± 0.26 IU mL-1); and for the activity of Xylanases, it was observed a higher activity at neutral pH (4.03 ± 0.20 IU mL-1).The enzymatic hydrolysis of the CR and JE flours, also promoted the increase of about 30% of phenolic compounds and total soluble sugars, with 48 h of exposure to enzymatic extract, which demonstrated that the enzymatic extract was efficient. This fact suggests the use of this extract for hydrolysis for enrichment food.