Avaliação da atividade antimicrobiana do polihexametileno biguanida (PHMB) em bactérias isoladas de fígados de frangos

Abstract

Chicken meat, due to its nutritional properties, requires high-quality standards to meet consumer expectations and mitigate risks associated with opportunistic microorganisms. In light of the growing search for alternatives to the indiscriminate use of antimicrobials, Polyhexamethylene Biguanide (PHMB) stands out as a surfactant agent effective against such microorganisms. This study aimed to evaluate the bacterial resistance profile and the antimicrobial activity of PHMB against bacteria isolated from broiler livers. To achieve this, the antimicrobial sensitivity was assessed using the disc diffusion method on Mueller Hinton agar. The Minimum Inhibitory Concentration (MIC) was determined through antimicrobial activity testing by dilution in a 96-well polystyrene plate, while the Minimum Biocidal Concentration (MBC) was confirmed at specific concentrations. Antibiogram results indicated a marked resistance to the tested antimicrobials. In the antimicrobial activity test, an inhibitory effect was observed across all tested concentrations, with biocidal confirmation at select concentrations. The consistency between the study's data and the literature reinforces the validity of the results. It is concluded that PHMB, at low concentrations, exhibits a mild inhibitory effect on the growth of bacteria isolated from poultry livers. However, further studies are required to confirm these findings.