ICB - Trabalhos de Conclusão de Curso
URI Permanente para esta coleção
Navegar
Navegando ICB - Trabalhos de Conclusão de Curso por Assunto "Adenovírus humano"
Agora exibindo 1 - 1 de 1
Resultados por página
Opções de Ordenação
Item Padronização de PCR multiplex para as regiões hexon penton e fibra de adenovírus humano(Universidade Federal de Goias, 2024-08-02) Sebata, Mayumi Ribeiro; Souza, Menira Borges de Lima Dias e; Souza, Menira Borges de Lima Dias e; Carneiro, Megmar Aparecida dos Santos; Freitas, Erika Regina Leal de; Porto, Pedro SoaresHuman adenoviruses (HAdV) are members of the Adenoviridae family classified withinthe genus Mastadenovirus. Within this genus, there are seven species of adenovirusesidentified from A to G, comprising over 100 known genotypes. Adenoviruses arenon-enveloped viruses with an icosahedral capsid primarily composed of hexon, penton,and fiber proteins. HAdV can cause respiratory, gastrointestinal, and ocular infections,manifesting symptoms such as fever, vomiting, and diarrhea, varying in severity basedon the host's immune system. Multiplex Polymerase Chain Reaction (PCR) utilizesmultiple primer pairs in a single reaction, noted for its efficiency and cost-effectiveness.Thus, multiplex PCR represents an effective alternative for enhancing research andlaboratory diagnosis of adenovirus infections. This study aims to standardize andvalidate a multiplex PCR assay for detecting hexon, penton, and fiber regions of humanadenoviruses across various sample types. Primer pairs were designed to target thesegenome regions in control samples of adenovirus species 2 cultured in A-549 cells andin samples from patients who underwent allogeneic hematopoietic progenitor celltransplantation and were previously positive for HAdV. Standardization of the multiplexPCR assay for penton and fiber genes was successful; however, amplifying the hexongene proved more challenging. The data obtained in this study will serve as a tool forfuture investigations into adenovirus recombination regions and deeper genomicanalyses of adenoviruses.Versão GRT_BC_maio