Padronização de PCR multiplex para as regiões hexon penton e fibra de adenovírus humano
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Data
2024-08-02
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Universidade Federal de Goias
Resumo
Human adenoviruses (HAdV) are members of the Adenoviridae family classified withinthe genus Mastadenovirus. Within this genus, there are seven species of adenovirusesidentified from A to G, comprising over 100 known genotypes. Adenoviruses arenon-enveloped viruses with an icosahedral capsid primarily composed of hexon, penton,and fiber proteins. HAdV can cause respiratory, gastrointestinal, and ocular infections,manifesting symptoms such as fever, vomiting, and diarrhea, varying in severity basedon the host's immune system. Multiplex Polymerase Chain Reaction (PCR) utilizesmultiple primer pairs in a single reaction, noted for its efficiency and cost-effectiveness.Thus, multiplex PCR represents an effective alternative for enhancing research andlaboratory diagnosis of adenovirus infections. This study aims to standardize andvalidate a multiplex PCR assay for detecting hexon, penton, and fiber regions of humanadenoviruses across various sample types. Primer pairs were designed to target thesegenome regions in control samples of adenovirus species 2 cultured in A-549 cells andin samples from patients who underwent allogeneic hematopoietic progenitor celltransplantation and were previously positive for HAdV. Standardization of the multiplexPCR assay for penton and fiber genes was successful; however, amplifying the hexongene proved more challenging. The data obtained in this study will serve as a tool forfuture investigations into adenovirus recombination regions and deeper genomicanalyses of adenoviruses.Versão GRT_BC_maio
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Adenovírus humano, Reação em cadeia da polimerase, Reação multiplex, Human adenovirus, Polymerase chain reaction, Multiplex reaction
Citação
SEBATA, Mayumi Ribeiro. Padronização de PCR multiplexpara as regiões hexon penton e fibra de adenovírus humano. 2024. 35 f. Trabalho de Conclusão de Curso (Bacharelado em Biomedicina) - Instituto de Ciências Biológicas, Universidade Federal de Goiás, Goiânia, 2024.