Cytotoxicity of glass ionomer cements containing silver nanoparticles
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Data
2015
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Resumo
Background: Some studies have investigated the possibility of incorporating silver nanoparticles (NAg) into dental
materials to improve their antibacterial properties. However, the potential toxic effect of this material on pulp cells
should be investigated in order to avoid additional damage to the pulp tissue. This study evaluated the cytotoxicity
of conventional and resin-modified glass ionomer cements (GIC) with and without addition of NAg.
Material and Methods: NAg were added to the materials at two different concentrations by weight: 0.1% and 0.2%.
Specimens with standardized dimensions were prepared, immersed in 400 μL of culture medium and incubated at
37°C and 5% CO2 for 48 h to prepare GIC liquid extracts, which were then incubated in contact with cells for 48 h.
Culture medium and 0.78% NAg solution were used as negative and positive controls, respectively. Cell viability
was determined by MTT and Trypan Blue assays. ANOVA and the Tukey test (α=0.05) were used for statistical
analyses.
Results: Both tests revealed a significant decrease in cell viability in all groups of resin modified cements (p˂0.001).
There were no statistically significant differences between groups with and without NAg (p˃0.05). The differences in cell
viability between any group of conventional GIC and the negative control were not statistically significant (p>0.05).
Conclusions: NAg did not affect the cytotoxicity of the GIC under evaluation.
Descrição
Palavras-chave
Glass ionomer cements, Totoxicity, Cell culture techniques, Nanotechnology, Metal nanoparticles
Citação
SIQUEIRA, Patrícia Correia; MAGALHÃES, Ana Paula Rodrigues; PIRES, Wanessa Carvalho; PEREIRA, Flávia Castro; LACERDA, Elisângela Paula Silveira; CARRIÃO, Marcus Santos; BAKUZIS, Andris Figueiroa; COSTA, Carlos Alberto Souza; LOPES, Lawrence Gonzaga; ESTRELA, Carlos. Cytotoxicity of glass ionomer cements containing silver nanoparticles. Journal of Clinical and Experimental Dentistry, Valência, v. 7, n. 5, p. 622-627, 2015.