Peptaibols from Trichoderma asperellum TR356 strain isolated from brazilian soil
Carregando...
Data
2014
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
The Trichoderma genus consists of a group of free-living filamentous fungi, including species able to act as biological
control agents (BCAs) against pathogenic fungi. It is believed that this ability is due to synergy between several
mechanisms, including the production of a wide variety of secondary metabolites by these organisms. Among
these, we highlight the production of peptaibols, an antibiotic peptide group characterized by the presence of
non-proteinogenic amino acids such as α-aminoisobutyrate (Aib), as well as by N-terminal modifications and
amino alcohols in the C-terminal region. This study aimed to outline a profile of peptaibol production and to
identify secreted peptaibols from the Trichoderma asperellum TR356 strain, described as an efficient BCA against
S. sclerotiorum. The fungus was grown on TLE 0.3% glucose medium for 5 days, with agitation at 120 rpm in the
dark. Liquid medium filtrate was used as the metabolite source. These extracts were subjected to high performance liquid
chromatography (HPLC) and subsequent analysis by matrix-assisted laser desorption ionization mass spectrometry
(MALDI-TOF). The results indicate the production of two classes of peptaibols for this T. asperellum strain. Primary
structures of two asperelines (A and E) and five trichotoxins (T5D2, T5E, T5F, T5G and 1717A) have been elucidated.
Most of these peptaibols had been previously described in T. viride and T. asperellum marine strains. This is the
first report of some of these compounds being produced by a T. asperellum strain from soil. Future analyses will
be necessary to elucidate the three-dimensional structures and their activities against pathogens.
Descrição
Palavras-chave
Trichoderma asperellum, Asperelines, Peptaibols, Trichotoxins, Mass spectrometry
Citação
BRITO, João Paulo Cabral de; RAMADA, Marcelo H. S.; MAGALHÃES, Mariana T. Q. de; SILVA, Luciano P.; ULHOA, Cirano J. Peptaibols from Trichoderma asperellum TR356 strain isolated from brazilian soil. SpringerPlus, London, v. 3, n. 600, p. 1-10, 2014.