Análise morfológica e microbiológica utilizando-se diferentes métodos de preservação sobre a camada muscular do intestino delgado de cães

Resumo

40 samples of the small intestines’ muscular layer from dogs were analyzed, maintained in different solutions preserved, being the solution 1: 98% glycerin, solution 2: tiomersal alcoholic dye at 1:1000, solution 3: 0,9% physiologic solution frozen at -16oC with antibiotic, solution 4: 0,9% physiologic solution frozen at -16oC, solution 5: 50% polivinilpirrolidone, solution 6: supersaturated sugar solution at 300% concentration and solution 7: supersaturated sugar solution at 300% concentration with antibiotic. The samples were kept in the preservation media for a 45 day period. After this period, they were submitted to microbiological analysis and evaluated by their macroscopic and histological aspects, and by HL Image 97++ image analyzer. There was no microorganism growth in any of the analyzed media. Both 98% glycerin solution and supersaturated sugar solution at 300% concentration, with or without the presence of antibiotic, were considered to be the most effective preservants, as they accomplished to maintain cellular integrity.

Descrição

Palavras-chave

Integridade celular, Preservação, Análise microbiológica, Cellular integrity, Preservation, Analysis microbiológical

Citação

MOTA, Francisco Cláudio Dantas; EURIDES, Duvaldo; FREITAS, Patrícia Maria Coletto; BELETTI, Marcelo Emílio; MASTRANTONIO, Eneida César; SHIMIZU, Bianca Jacob; SILVA, Luiz Antônio Franco da; CARDOSO, Júlio Roquete; MARTINS, Alan Kardec. Análise morfológica e microbiológica utilizando-se diferentes métodos de preservação sobre a camada muscular do intestino delgado de cães. Ciência Animal Brasileira, Goiânia, v. 4, n. 2, p. 117-123, jul./dez. 2003. Disponível em: <https://www.revistas.ufg.br/vet/article/view/308/276>.