Análise morfológica e microbiológica utilizando-se diferentes métodos de preservação sobre a camada muscular do intestino delgado de cães
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Data
2003-12
Título da Revista
ISSN da Revista
Título de Volume
Editor
José Henrique Stringhini
Resumo
40 samples of the small intestines’ muscular layer
from dogs were analyzed, maintained in different solutions
preserved, being the solution 1: 98% glycerin, solution 2:
tiomersal alcoholic dye at 1:1000, solution 3: 0,9%
physiologic solution frozen at -16oC with antibiotic, solution
4: 0,9% physiologic solution frozen at -16oC, solution 5:
50% polivinilpirrolidone, solution 6: supersaturated sugar
solution at 300% concentration and solution 7:
supersaturated sugar solution at 300% concentration with
antibiotic. The samples were kept in the preservation media
for a 45 day period. After this period, they were submitted
to microbiological analysis and evaluated by their
macroscopic and histological aspects, and by HL Image
97++ image analyzer. There was no microorganism growth in
any of the analyzed media. Both 98% glycerin solution and
supersaturated sugar solution at 300% concentration, with
or without the presence of antibiotic, were considered to be
the most effective preservants, as they accomplished to
maintain cellular integrity.
Descrição
Palavras-chave
Integridade celular, Preservação, Análise microbiológica, Cellular integrity, Preservation, Analysis microbiológical
Citação
MOTA, Francisco Cláudio Dantas; EURIDES, Duvaldo; FREITAS, Patrícia Maria Coletto; BELETTI, Marcelo Emílio; MASTRANTONIO, Eneida César; SHIMIZU, Bianca Jacob; SILVA, Luiz Antônio Franco da; CARDOSO, Júlio Roquete; MARTINS, Alan Kardec. Análise morfológica e microbiológica utilizando-se diferentes métodos de preservação sobre a camada muscular do intestino delgado de cães. Ciência Animal Brasileira, Goiânia, v. 4, n. 2, p. 117-123, jul./dez. 2003. Disponível em: <https://www.revistas.ufg.br/vet/article/view/308/276>.