Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver
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Data
2012-10
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Resumo
The hypoferremia that is observed during systemic in lammatory processes is mediated
by hepcidin, which is a peptide that is mainly synthesized in the livers of several mammalian
species. Hepcidin plays a key role in iron metabolism and in the innate immune system.
It’s up-regulation is particularly useful during acute in lammation, and it restricts the iron
availability that is necessary for the growth of pathogenic microorganisms. In this study,
the hepcidin mRNA of Equus asinus has been characterized, and the expression of donkey
hepcidin in the liver has been determined. The donkey hepcidin sequence has an open
reading frame (ORF) of 261 nucleotides, and the deduced corresponding protein sequence
has 86 amino acids. The amino acid sequence of donkey hepcidin was most homologous to
Equus caballus (98%). The mature donkey hepcidin sequence (25 amino acids) was 100%
homologous to the equine mature hepcidin and has eight conserved cysteine residues that
are found in all of the investigated hepcidin sequences. The expression pro ile of donkey
hepcidin in the liver was high and was similar to the reference gene expression. The donkey
hepcidin sequence was deposited in GenBankTM (HQ902884) and may be useful for additional
studies on iron metabolism and the in lammatory process in this species.
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Hepcidin, mRNA, Sequencing, Expression, Equus asinus, Hepcidina, Sequenciamento e expressão gênica
Citação
OLIVEIRA FILHO, José P.; MARQUES, Jessica A.; CUNHA, Paulo Henrique J.; MEDEIROS, Gildenor X.; RIET-CORREA, Franklin; MACHADO, Vânia Maria V.; BORGES, Alexandre S. Sequencing and expression analysis of hepcidin mRNA in donkey (Equus asinus) liver. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 32, n. 10, p. 1050-1054, Oct. 2012.