Antinociceptive, anti-inflammatory and anxiolytic-like effects of the ethanolic extract, fractions and Hibalactone isolated from Hydrocotyle umbellata L. (Acariçoba) – Araliaceae
Nenhuma Miniatura disponível
Data
2017
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Hydrocotyle umbellata Linn. (Araliaceae) is specie used in the treatment of inflammatory diseases. Crude extract
(E-HU) was prepared from H. umbellata subterraneous parts and fractionated by liquid-liquid partition, resulting
hexane fraction (HF-HU), dichloromethane fraction (DF-HU), ethyl acetate fraction (EAF-HU) and aqueous
fraction (AF-HU). The hibalactone (HU-1) was isolated from the DF-HU and its structure was elucidated by 1H
NMR and 13C NMR Spectroscopy, mass spectrometry and crystallographic x-ray analysis. The formalin-induced
nociception was used to evaluate antinociceptive activity; carrageenan-induced edema and pleurisy tests to
evaluate anti-inflammatory activity and light-dark box to evaluate anxiolytic-like activity in mice. The acute oral
treatments with E-HU (1000 mg/kg), DF-HU (150 mg/kg), EAF-HU (400 mg/kg) and HU-1 (33 mg/kg) decreased
the licking time in both phases of the formalin test. In the carrageenan-induced inflammation models,
the treatment with the same doses of E-HU, DF-HU, EAF-HU and HU-1 reduced the paw edema formation and
leukocytes account into pleural cavity. In silico findings suggest that hibalactone present anti-inflammatory
activity by interacting with the enzymes 5-lipoxygenase and cyclooxygenase-2. In the light dark box, the
treatments with DF-HU, EAF-HU and HU-1 revealed an anxiolytic like effect. Thus, the E-HU and fractions of H.
umbellata showed antinociceptive, anti-inflammatory and anxiolytic like activities, as also hibalactone, a possible
phytoconstituent responsible for the biological effects of this specie.
Descrição
Palavras-chave
Hibalactone, Medicinal plants, Hydrocotyle umbellata L., Anti-inflammatory, Anxiolytic-like, Antinociceptive
Citação
OLIVEIRA,Thiago Levi Silva et al. Antinociceptive, anti-inflammatory and anxiolytic-like effects of the ethanolic extract, fractions and Hibalactone isolated from Hydrocotyle umbellata L. (Acariçoba) - Araliaceae. Biomedicine & Pharmacotherapy, New York, v. 95, p. 837-846, 2017. DOI: 10.1016/j.biopha.2017.08.140. Disponível em: https://www.sciencedirect.com/science/article/pii/S0753332217320139?via%3Dihub. Acesso em: 27 abr. 2023.