Acomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110

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dc.creatorPennacchi, Mariana Gomes Clemente
dc.creatorRodríguez Fernández, Daniel Ernesto
dc.creatorVendruscolo, Francielo
dc.creatorMaranho, Leila Teresinha
dc.creatorMarc, Ivan
dc.creatorCardoso, Lígia Alves da Costa
dc.date.accessioned2020-10-05T15:40:31Z
dc.date.available2020-10-05T15:40:31Z
dc.date.issued2015-03
dc.description.abstractCarotenoids are intracellular pigments produced by microorganisms, including some species of yeasts, in the stationary phase of growth by the secondary metabolic pathways. In the present study, different methods of Sporobolomyces ruberrimus H110 yeast cell lysis were evaluated with the objective of optimizing the recovery of intracellular pigments. Three extraction methods were used: vortex (glass beads and quartz stones), planetary mill (glass beads and quartz stones) and dimethyl sulfoxide. For each one of the lysis agents studied, factorial designs were developed considering as independent variables the agitation speed and lysis agent concentration. A central composite planning was defined considering as independent variables the lysis agent concentration and agitation speed, analyzing as response the estimated total number of extracted carotenoids. From the methods studied, a better extraction of total carotenoid (1.74 mg.g-1 of cells and of 1.57 mg.g-1 of cells) using the planetary mill method with 135 mg of glass beads or irregular quartz stones, with an agitation speed of 200 rpm. As to the cell lysis, the analysis indicated that the mechanical methods studied showed to be efficient in regards to cell laceration.pt_BR
dc.identifier.citationPENNACCHI, Mariana Gomes Clemente et al. Acomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110. International Journal of Applied Biology and Pharmaceutical Technology, Houston, v. 6, n. 1, p. 136-143, 2015.pt_BR
dc.identifier.issne- 0976-4550
dc.identifier.urihttp://repositorio.bc.ufg.br/handle/ri/19139
dc.language.isoengpt_BR
dc.publisher.countryEstados unidospt_BR
dc.publisher.departmentEscola de Agronomia - EA (RG)pt_BR
dc.rightsAcesso Abertopt_BR
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/br/*
dc.subjectYeastpt_BR
dc.subjectCarotenoidspt_BR
dc.subjectCell lysispt_BR
dc.subjectExtractionpt_BR
dc.subjectPlanetary millpt_BR
dc.titleAcomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110pt_BR
dc.typeArtigopt_BR

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