Acomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110
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Data
2015-03
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Resumo
Carotenoids are intracellular pigments produced by microorganisms, including some species of
yeasts, in the stationary phase of growth by the secondary metabolic pathways. In the present study, different
methods of Sporobolomyces ruberrimus H110 yeast cell lysis were evaluated with the objective of optimizing
the recovery of intracellular pigments. Three extraction methods were used: vortex (glass beads and quartz
stones), planetary mill (glass beads and quartz stones) and dimethyl sulfoxide. For each one of the lysis agents
studied, factorial designs were developed considering as independent variables the agitation speed and lysis
agent concentration. A central composite planning was defined considering as independent variables the lysis
agent concentration and agitation speed, analyzing as response the estimated total number of extracted
carotenoids. From the methods studied, a better extraction of total carotenoid (1.74 mg.g-1 of cells and of
1.57 mg.g-1 of cells) using the planetary mill method with 135 mg of glass beads or irregular quartz stones,
with an agitation speed of 200 rpm. As to the cell lysis, the analysis indicated that the mechanical methods
studied showed to be efficient in regards to cell laceration.
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Yeast, Carotenoids, Cell lysis, Extraction, Planetary mill
Citação
PENNACCHI, Mariana Gomes Clemente et al. Acomparison of cell disruption procedures for the recovery of intracellular carotenoids from Sporobolomyces ruberrimus H110. International Journal of Applied Biology and Pharmaceutical Technology, Houston, v. 6, n. 1, p. 136-143, 2015.