Cell wall synthesis, development of hyphae and metabolic pathways are processes potentially regulated by microRNAs produced between the morphological stages of Paracoccidioides brasiliensis
Nenhuma Miniatura disponível
Data
2018
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
MicroRNAs are molecules involved in post-transcriptional gene regulation. In pathogenic
fungi, microRNAs have been described at different morphological stages by regulating
targets involved in processes such as morphogenesis and energy production. Members
of the Paracoccidioides complex are the main etiological agents of a systemic mycosis in
Latin America. Fungi of the Paracoccidioides complex present a wide range of plasticity
to colonize different niches. In response to environmental changes these fungi undergo
a morphological switch, remodel their cellular metabolism and modulate structural cell
wall components. However, the underlying mechanisms regulating the gene expression
is not well understood. By using high performance sequencing and bioinformatics
analyses, this work characterizes microRNAs produced by Paracoccidioides brasiliensis.
Here, we demonstrated that the transcript encoding proteins involved in microRNA
biogenesis were differentially expressed in each morphological stage. In addition, 49
microRNAs were identified in cDNA libraries with 44 differentially regulated among
the libraries. Sixteen microRNAs were differentially regulated in comparison to the
mycelium in the mycelium-to-yeast transition phase. The yeast parasitic phase revealed
a complete remodeling of the expression of these small RNAs. Analyses of targets of
the induced microRNAs, from the different libraries, revealed that these molecules may
potentially regulate in the cell wall, by repressing genes involved in the synthesis and
degradation of glucans and chitin. Furthermore, mRNAs involved in cellular metabolism
and development were predicted to be regulated by microRNAs. Therefore, this
work describes a putative post transcriptional regulation, mediated by microRNAs in
P. brasiliensis and its influence on the adaptive processes of thermal dimorphic fungus.
Descrição
Palavras-chave
Paracoccidioides, MicroRNAs, PCM, Cell wall, Mycosis
Citação
CURCIO, Juliana S. de et al. Cell wall synthesis, development of hyphae and metabolic pathways are processes potentially regulated by microRNAs produced between the morphological stages of Paracoccidioides brasiliensis. Frontiers in Microbiology, Lausanne, v. 9, e3057, 2018. DOI: 10.3389/fmicb.2018.03057. Disponível em: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.03057/full. Acesso em: 25 nov. 2024.