Cell wall synthesis, development of hyphae and metabolic pathways are processes potentially regulated by microRNAs produced between the morphological stages of Paracoccidioides brasiliensis

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dc.creatorCurcio, Juliana Santana de
dc.creatorPaccez, Juliano Domiraci
dc.creatorNovaes, Evandro
dc.creatorBrock, Mathias
dc.creatorSoares, Célia Maria de Almeida
dc.date.accessioned2024-11-28T15:07:52Z
dc.date.available2024-11-28T15:07:52Z
dc.date.issued2018
dc.description.abstractMicroRNAs are molecules involved in post-transcriptional gene regulation. In pathogenic fungi, microRNAs have been described at different morphological stages by regulating targets involved in processes such as morphogenesis and energy production. Members of the Paracoccidioides complex are the main etiological agents of a systemic mycosis in Latin America. Fungi of the Paracoccidioides complex present a wide range of plasticity to colonize different niches. In response to environmental changes these fungi undergo a morphological switch, remodel their cellular metabolism and modulate structural cell wall components. However, the underlying mechanisms regulating the gene expression is not well understood. By using high performance sequencing and bioinformatics analyses, this work characterizes microRNAs produced by Paracoccidioides brasiliensis. Here, we demonstrated that the transcript encoding proteins involved in microRNA biogenesis were differentially expressed in each morphological stage. In addition, 49 microRNAs were identified in cDNA libraries with 44 differentially regulated among the libraries. Sixteen microRNAs were differentially regulated in comparison to the mycelium in the mycelium-to-yeast transition phase. The yeast parasitic phase revealed a complete remodeling of the expression of these small RNAs. Analyses of targets of the induced microRNAs, from the different libraries, revealed that these molecules may potentially regulate in the cell wall, by repressing genes involved in the synthesis and degradation of glucans and chitin. Furthermore, mRNAs involved in cellular metabolism and development were predicted to be regulated by microRNAs. Therefore, this work describes a putative post transcriptional regulation, mediated by microRNAs in P. brasiliensis and its influence on the adaptive processes of thermal dimorphic fungus.
dc.identifier.citationCURCIO, Juliana S. de et al. Cell wall synthesis, development of hyphae and metabolic pathways are processes potentially regulated by microRNAs produced between the morphological stages of Paracoccidioides brasiliensis. Frontiers in Microbiology, Lausanne, v. 9, e3057, 2018. DOI: 10.3389/fmicb.2018.03057. Disponível em: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.03057/full. Acesso em: 25 nov. 2024.
dc.identifier.doi10.3389/fmicb.2018.03057
dc.identifier.issne- 1664-302X
dc.identifier.urihttp://repositorio.bc.ufg.br//handle/ri/26015
dc.language.isoeng
dc.publisher.countrySuica
dc.publisher.departmentInstituto de Ciências Biológicas - ICB (RMG)
dc.rightsAcesso Aberto
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectParacoccidioides
dc.subjectMicroRNAs
dc.subjectPCM
dc.subjectCell wall
dc.subjectMycosis
dc.titleCell wall synthesis, development of hyphae and metabolic pathways are processes potentially regulated by microRNAs produced between the morphological stages of Paracoccidioides brasiliensis
dc.typeArtigo

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