Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep
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Data
2015-09
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Resumo
In order to detect virulence factors in Shiga toxin-producing Escherichia coli (STEC) isolates
and investigate the antimicrobial resistance profile, rectal swabs were collected from healthy
sheep of the races Santa Inês and Dorper. Of the 115 E. coli isolates obtained, 78.3% (90/115)
were characterized as STEC, of which 52.2% (47/90) carried stx1 gene, 33.3% (30/90) stx2
and 14.5% (13/90) both genes. In search of virulence factors, 47.7% and 32.2% of the isolates
carried the genes saa and cnf1. According to the analysis of the antimicrobial resistance
profile, 83.3% (75/90) were resistant to at least one of the antibiotics tested. In phylogenetic
classification grouped 24.4% (22/90) in group D (pathogenic), 32.2% (29/90) in group B1
(commensal) and 43.3% (39/90) in group A (commensal). The presence of several virulence
factors as well as the high number of multiresistant isolates found in this study support the
statement that sheep are potential carriers of pathogens threatening public health.
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Escherichia coli, STEC, Multiresistant isolates, Molecular characterization, Phylogenetic, Escherichia coli,, Isolados multirresistentes, Caracterização molecular, Filogenético
Citação
FERREIRA, Marcos R. A. et al. Detection of virulence factors and antimicrobial resistance patterns in shiga toxin-producing Escherichia coli isolates from sheep. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 35, n. 9, p. 775-780, Sept. 2015.