Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil
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Data
2008-12
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Resumo
Hepatitis A virus (HAV) infection is a public health problem worldwide and the virus has been classified into six
genotypes. In Brazil, the only genotype that has been found is genotype I, predominately from subgenotype IA. Here,
the HAV genotypes were analyzed of 18 isolates circulating between 1996-2001 in Goiânia, state of Goiás, Brazil.
Viral RNA was extracted from 18 serum samples and amplified (RT-PCR/nested-PCR), followed by the genomic
sequencing of the VP1/2A junction region of the HAV genome. Sequences of 168 nucleotides were compared and
analyzed using the BLAST N, Clustal X and PAUP v. 4.10b programs. All samples were classified as genotype I, with
10 belonging to subgenotype IA and eight to subgenotype IB. The subgenotype IA isolates showed greater diversity
than the subgenotype IB isolates at the nucleotide level. Elevated identity values were found between isolates ob-
tained in this study and those from other regions of the world, including Brazil, highlighting the high conservation
among different isolates of this virus. However, changes in the HAV subgenotype circulation could also be observed
during the evaluated period.
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Acute hepatitis, Subgenotype IB, Nucleotide sequencing, Subgenotype IA
Citação
FIACCADORI, Fabíola Souza; PEREIRA, Maristela ; COELHO, Alexandre Siqueira Guedes; BORGES, Ana Maria Tavares; PARENTE, Juliana Alves; SOARES, Célia Maria de Almeida; CARDOSO, Divina das Dores de Paula. Molecular characterization of hepatitis A virus isolates from Goiânia, Goiás, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 103, n. 8, p. 831-835, Dec. 2008.