Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV
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Data
2004-09
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Resumo
The oral dosages formulations from Pothomorphe umbellata
(Piperaceae) can only be therapeutically evaluated after
establishing 4-nerolidylcatechol (4-NRC) pharmacokinetic profile
in plasma. For that purpose, a unique analytical method validation
using HPLC-UV detection was developed for analysis of rat plasma
samples. The animals received 10 mg/kg b.w. of 4-NRC i.v..
Analytical conditions were set with C18 column, methanol:
acetonitrile: water (62:20:18) as mobile phase, and a flow rate of
1.6 mL/min. The assay was linear from 1.0 - 80.0 mcg/mL. The
recovery procedure was performed by liquid-liquid extraction
showing quantitative extraction (106.4±8.7%). Limit of
Quantification (LOQ) was found to be 1.0 mcg/mL. Interassay
precision and accuracy ranged from values of 14.4-3.0% and 24.0–
0.2%, respectively. Recovery after liquid-liquid extraction was found
to be 106.5 ± 3%. The method is simple and reliable with total run
time of less than 15 min.
Descrição
Palavras-chave
4-Nerolidylcatechol, Pothomorphe umbellata, HPLC-UV, Liquid-liquid extraction, 4-Nerolidilcatecol, Extração líquido-líquido, Pothomorphe umbellata, CLAE-UV
Citação
REZENDE, Kênnia Rocha; BARROS, Sílvia Berlanga de Moraes. Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV. Revista Brasileira de Ciências Farmacêuticas, São Paulo, v. 40, n. 3, p. 373-380, Jul./Sept. 2004.