Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV
| dc.creator | Rezende, Kênnia Rocha | |
| dc.creator | Barros, Silvia Berlanga de Moraes | |
| dc.date.accessioned | 2019-02-13T14:53:28Z | |
| dc.date.available | 2019-02-13T14:53:28Z | |
| dc.date.issued | 2004-09 | |
| dc.description.abstract | The oral dosages formulations from Pothomorphe umbellata (Piperaceae) can only be therapeutically evaluated after establishing 4-nerolidylcatechol (4-NRC) pharmacokinetic profile in plasma. For that purpose, a unique analytical method validation using HPLC-UV detection was developed for analysis of rat plasma samples. The animals received 10 mg/kg b.w. of 4-NRC i.v.. Analytical conditions were set with C18 column, methanol: acetonitrile: water (62:20:18) as mobile phase, and a flow rate of 1.6 mL/min. The assay was linear from 1.0 - 80.0 mcg/mL. The recovery procedure was performed by liquid-liquid extraction showing quantitative extraction (106.4±8.7%). Limit of Quantification (LOQ) was found to be 1.0 mcg/mL. Interassay precision and accuracy ranged from values of 14.4-3.0% and 24.0– 0.2%, respectively. Recovery after liquid-liquid extraction was found to be 106.5 ± 3%. The method is simple and reliable with total run time of less than 15 min. | pt_BR |
| dc.description.resumo | O 4-nerolidilcatecol representa o metabólito secundá- rio mais abundante de Pothomorphe umbellata (Piperaceae), cujo uso terapêutico deve ser fundamen- tado após a caracterização farmacocinética do mesmo (4-nerolidicatecol). Os níveis séricos de animais trata- dos com 10 mg/kg do fármaco i.v. foram avaliados por validação analítica em CLAE-UV. As condições cromatográficas empregadas foram fase móvel de acetonitrila:metanol:água (62:20:18), coluna RP-18, fluxo de 1,6 mL/min e detector UV em 282 nm, com tempo total de corrida inferior a 15 minutos. Observou-se linearidade do 4-NRC em plasmas na faixa de 1,0 a 80,0 mcg/mL, com valores de precisão e exatidão entre 14,4- 3,0% e 24,0–0,2%, respectivamente. O procedimento de extração líquido–líquido apresentou reprodutível e com recuperação quantitativa (106,5%±3,0%). | pt_BR |
| dc.identifier.citation | REZENDE, Kênnia Rocha; BARROS, Sílvia Berlanga de Moraes. Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV. Revista Brasileira de Ciências Farmacêuticas, São Paulo, v. 40, n. 3, p. 373-380, Jul./Sept. 2004. | pt_BR |
| dc.identifier.doi | 10.1590/S1516-93322004000300013 | |
| dc.identifier.issn | 1516-9332 | |
| dc.identifier.uri | http://repositorio.bc.ufg.br/handle/ri/17057 | |
| dc.language.iso | eng | pt_BR |
| dc.publisher.country | Brasil | pt_BR |
| dc.publisher.department | Faculdade de Farmácia - FF (RG) | pt_BR |
| dc.rights | Acesso Aberto | pt_BR |
| dc.subject | 4-Nerolidylcatechol | pt_BR |
| dc.subject | Pothomorphe umbellata | pt_BR |
| dc.subject | HPLC-UV | pt_BR |
| dc.subject | Liquid-liquid extraction | pt_BR |
| dc.subject | 4-Nerolidilcatecol | pt_BR |
| dc.subject | Extração líquido-líquido | pt_BR |
| dc.subject | Pothomorphe umbellata | pt_BR |
| dc.subject | CLAE-UV | pt_BR |
| dc.title | Quantification of 4-nerolidylcatechol from Pothomorphe umbellata (Piperaceae) in rat plasma samples by HPLC-UV | pt_BR |
| dc.title.alternative | Quantificação de 4-nerolidilcatecol de Pothomorphe umbellata (Piperaceae) em amostras de plasma de rato por CLAE-UV | pt_BR |
| dc.type | Artigo | pt_BR |