Development and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae)
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2020-10
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Resumo
In this study, we report the development and
characterization of 15 new microsatellite markers for
Stryphnodendron adstringens (Leguminosae) in order to
support future analyses of genetic diversity in populations
of this species. In screening with 48 individuals from three
different populations of S. adstringens, we tested the
amplification of 20 microsatellite loci, of which five are not
useful for population genetic studies due to the lack of
polymorphisms or amplification failures. For the final set of
15 loci, the number of alleles ranged from 2 to 15, with a
total of 116 alleles. The expected heterozygosity ranged
from 0.1219 to 0.8965, with an average of 0.6694 per
locus. The combined probability of genetic identity
(PI = 8.12 9 10-15) and paternity exclusion
(Q = 0.99999) estimations showed that the loci may be
useful to discriminate between individuals of S. adstringens.
Initial cross-amplification tests were satisfactory in
three species of the genus Stryphnodendron: S. rotundifolium, S. coriaceum and S. polyphyllum. This new
set of markers will be a useful tool for population genetic
studies, contributing to the knowledge about the evolutionary
history of S. adstringens and, additionally, correlated
species.
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Cerrado, SSR markers, Genetic diversity, Medicinal plant, Next generation sequencing
Citação
GONCALVES, Ariany Rosa; BARATELI, Luciana Oliveira; SOUZA, Ueric José Borges de; PEREIRA, Ana Maria Soares; BERTONI, Bianca Waléria; TELLES, Mariana Pires de Campos. Development and characterization of microsatellite markers in Stryphnodendron adstringens (Leguminosae). Physiology and Molecular Biology of Plants, Washington, v. 16, n. 10, p. 1-7, Oct. 2020. DOI: 10.1007/s12298-020-00876-1. Disponível em: https://www-ncbi-nlm-nih.ez49.periodicos.capes.gov.br/pmc/articles/PMC7548263/pdf/12298_2020_Article_876.pdf. Acesso em: 25 jan. 2023.